Journal ArticleDOI
RNA species obtained from clonal lines of avian sarcoma and from avian leukosis virus
TLDR
Gel electrophoresis of the dissociated 60–70 S RNA prepared from cloned avian sarcoma viruses showed a single major peak corresponding to size class a .About:
This article is published in Virology.The article was published on 1973-07-01. It has received 127 citations till now. The article focuses on the topics: Avian sarcoma virus & RNA.read more
Citations
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Journal ArticleDOI
Die Molekularbiologie der Tumorviren
TL;DR: All classes of vertebrates harbor tumor viruses that are capable of inducing either tumors or leukemias, and these viruses have already been assigned to specific regions of their DNA.
Journal ArticleDOI
Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sites.
Stephen H. Hughes,Peter R. Shank,Deborah H. Spector,Hsing Jien Kung,J. Michael Bishop,Harold E. Varmus,Peter K. Vogt,Martin L. Breitman +7 more
TL;DR: Assessment of DNA from 15 clones of avian sarcoma virus-transformed rat cells with restriction endonucleases and molecular hybridization techniques found that ASV DNA can be accommodated at many positions in cellular DNA, but the existence of preferred sites has not been excluded.
Journal ArticleDOI
Characterization of Rous sarcoma virus src gene products synthesized in vitro.
Karen Beemon,Tony Hunter +1 more
TL;DR: The cell-free synthesis of three major proteins from virion RNA of nondefective Rous sarcoma virus (RSV), but not from RNA of transformation-defective deletion mutants, has been observed and it is suggested that synthesis of the 60K protein is initiated near the 5' terminus of thesrc gene, whereas the 39K, 33K, 25K, and 17K proteins are initiated internally in the src gene.
Journal ArticleDOI
Molecular cloning and characterization of avian sarcoma virus circular DNA molecules.
TL;DR: Results demonstrate that the cloned DNA molecules are functionally identical to viral DNA produced in vivo; therefore, molecular cloning did not cause any major alterations of the DNA.
Book ChapterDOI
Cell Transformation by RNA Tumor Viruses
TL;DR: The establishment of an assay system for Rous sarcoma virus (RSV) in tissue culture cells (Temin and Rubin, 1958) eventually led to an era of quantitative studies on the mechanism of cellular alteration by viruses.
References
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Journal Article
Application of a microtechnique to viral serological investigations.
TL;DR: A microtechnique (modified Takatsy) is described which can be applied to complement fixation, hemagglutination, hemagenesis inhibition and metabolic inhibition tests andComparative data obtained with the micro- and standard systems establish the reliability and validity of the microsystem.
Journal ArticleDOI
Induction of avian tumor viruses in normal cells by physical and chemical carcinogens.
TL;DR: The chromosomal locus which controls the presence of natural gs antigen in chicken cells does not represent the viral genome itself but regulates its expression in normal cells, which has implications for the origin of RNA tumor viruses and for theories of carcinogenesis.
Journal ArticleDOI
Genetic recombinants and heterozygotes derived from endogenous and exogenous avian RNA tumor viruses
TL;DR: Genotypic mixing was not evident in chf -negative cells which contain viral DNA but not viral RNA, suggesting that genetic reassortment occurs among RNA molecules, and a model is proposed in which reassortment of independent genome segments may be converted into stable recombinants following provirus formation in the next replicative cycle.
Journal ArticleDOI
Differences between the Ribonucleic Acids of Transforming and Nontransforming Avian Tumor Viruses
Peter H. Duesberg,Peter K. Vogt +1 more
TL;DR: The 60–70S RNAs of several transforming and nontransforming avian tumor viruses have different electrophoretic mobilities and it is suggested that the presence of the class a subunit is related to the transforming ability for fibroblasts of the virus.
Related Papers (5)
Differences between the Ribonucleic Acids of Transforming and Nontransforming Avian Tumor Viruses
Peter H. Duesberg,Peter K. Vogt +1 more