Showing papers in "Cell in 1978"

TL;DR: The experiments established the usefulness of the bybrid myeloma technique in preparing monospecific antibodies against human cell surface antigens and highlights the possibilities not only of obtaining reagents for somatic cell genetics, but also of obtaining mouse antibodies detecting human antigenic polymorphisms.

TL;DR: A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.

TL;DR: Using one embryonal carcinoma cell line, F9, it is found that retinoic acid at concentrations as low as 10(-9) M induces multiple phenotypic changes in the cultures in vitro, which are consistent with the proposition that retinosic acid induces differentiation of embryonal cancer cells into endoderm.

TL;DR: Dye-coupling, readily observed because of the low molecular weight and the intense fluorescence of the new dye, may serve as a general method of tracing certain functional connections by morphological means, and of studying the transfer of small molecules between cells.

TL;DR: High molecular weight DNA from cells containing only one copy of the HSV gene coding for tk was successfully used to transform L+K-cells to the tk+ phenotype, and the acquired phenotype was demonstrated to be donor-derived by analysis of the electrophoretic mobility of the tK activity.

TL;DR: Butyrate appears to be an inhibitor of histone deacetylases both in vivo and in vitro, and induces the synthesis of a nonhistone chromosomal protein, IP25 in Friend erythroleukemic cells.

TL;DR: Secretion of infectious Abelson leukemia virus by two of the cloned cell lines provides conclusive evidence that the Abelson virus is capable of productively infecting the macrophage cell type.

TL;DR: The two independently isolated β-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the δ- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence.

TL;DR: Assessment of present knowledge about the mechanism by which eucar- yotic ribosomes select the correct site for initiation of protein synthesis finds certain features of translational initia- tion in eucaryotes are sufficiently compelling to warrant discussion.

TL;DR: Erythrocyte ghosts containing a known number of molecules of purified fragment A of diphtheria toxin with a constant amount of FITC-BSA as a fluorescence marker demonstrated that a single molecule of fragment A was sufficient to kill a cell.

TL;DR: It is likely that PDGF is released from platelets at sites of vascular damage and that it contributes toward the cell proliferation and connective tissue formation seen in healing wounds and in arteriosclerotic lesions.

TL;DR: The hyperacetylation of histones due to treatment of cultured cells with sodium butyrate has been studied and it is found that 80% of histone H4 is acetylated after a 24 hr exposure tobutyrate.

TL;DR: It is concluded that both the protein kinase and pp60 are encoded in src, and that the enzymatic activity may be an intrinsic property of pp60.

TL;DR: The results suggest independent regulation of X 2 Y in this situation and suggest the loss of differentiated function by these chondrocyte progeny is indicated.

TL;DR: It is concluded that the microtubule assembly-disassembly "equilibrium" is a steady state summation of two different reactions which occur at opposite ends of the micro Tubule, and that assembly and disassembly occur predominantly and perhaps exclusively at the opposite ends under steady state conditions in vitro.

TL;DR: A simple and selective assay for monomeric and filamentous actin is presented, based on the inhibition of DNAase I by actin, which is rapid enough to detect changes in the polymerization state of actin in vitro over time intervals as short as 3 min.

TL;DR: It is proposed that a Q beta phage population is in a dynamic equilibrium, with viable mutants arising at a high rate (Batschelet, Domingo and Weissmann, 1976) on the one hand, and being strongly selected against on the other.

TL;DR: The structure of adenovirus 2 (Ad2) cytoplasmic RNAs produced during the early phase of infection is defined and it is suggested that, like the SV40 tumor antigens, the polypeptides encoded by these Ad2 mRNAs have an identical amino acid sequence at their N terminal ends, but have different C terminal sequences.

TL;DR: Outstanding research works that have attempted to ascertain the underlying epigenetic changes of the nuclear genome during in vitro differentiation of MSCs into bone and cartilage cell lineages are covered.

TL;DR: Several laboratories have reported synthesis of proteins with similar molecular weights in cells subjected to conditions that alter glucose metabolism, and it is speculated that these proteins may be associated with a hexose transport system.

TL;DR: The data presented are consistent with a stochastic model of differentiation for L 6 E 9 cells and demonstrate that the switch from a proliferating to a differentiating genetic program can occur in the absence of DNA synthesis.

TL;DR: A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2 as discussed by the authors.

TL;DR: Four agents known to increase the level of cellular cAMP by different means increase the growth of colonies of cultured human epidermal cells and of keratinocytes derived from other stratified squamous epithelia, suggesting that cell proliferation in the intact epidermis is regulated through agents affecting cAMP.

TL;DR: It is proposed that fibronectin may participate in the formation of attachment plaques and discuss the interrelationship between plaques, microfilament bundles and fibronECTin in cell-substratum and cell-cell contacts.

TL;DR: The nucleotide sequences of the germ line gene as well as a corresponding somatically mutated and rearranged gene coding for a mouse immunoglobulin lambdaI type light chain are determined and confirm the previously drawn conclusion that an active complete lambdaI gene arises by somatic recombination.

TL;DR: An antibody was raised against an electrophoretically homogeneous protein from cultured fibroblasts and shown to be directed against 10 nm filaments, and the antiserum did not stain microtubules or actin microfilaments.

TL;DR: The data are consistent with a mechanism in which this inhibitor activates a nuclease which prevents mRNA from being utilized for protein synthesis, and are contrasted with that of the heme-controlled repressor, another potent inhibitor of translation.

TL;DR: This catalog of proteins, combined with 50 additional ribosomal proteins already studied, comprises about 5% of the coding capacity of the genome, but accounts for two thirds of the cell's protein mass.

TL;DR: These data on conservation of the Ad2 sequences suggest that each transcriptional event from the 16-99 transcription unit gives rise to one of a possible 13-14 mRNA molecules with destruction of the remainder of the transcribed RNA.