Journal ArticleDOI
Sectioning refractory animal tissues.
I. Griffiths,Muriel E. Carter +1 more
TLDR
A method is described tor sectioning refractory material, particularly heavily yolked tissue, which has produced excellent sections and flaccid ribbons in a range of material which, prior to solvation, proved extremely Refractory and, in some instances, impossible to cut.Abstract:
A method is described tor sectioning refractory material, particularly heavily yolked tissue Fixation in a saturated picric acid-dioxan solution containing 10% formalin and 5% formic acid is recommended followed by dehydration in 2-ethoxyethanol This greatly reduces pre-embedding hardening Prior to clearing in three 4-hour changes of benzene, the tissue is placed for 12 hours in a bath of 2% celloidin in 2-ethoxyethanol This impregnation prevents subsequent imbibition pressure from disrupting the anatomical relationships of the different parts The material is embedded in wax and the block, trimmed to expose the tissue, is solvated for 12 hours in a 5% solution of Tergitol in pure ethane diol This method has produced excellent sections and flaccid ribbons in a range of material which, prior to solvation, proved extremely refractory and, in some instances, impossible to cutread more
Citations
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Evolution of reptilian placentation: Development of extraembryonic membranes of the Australian scincid lizards, Bassiana duperreyi (Oviparous) and Pseudemoia entrecasteauxii (Viviparous).
TL;DR: A revision of a classic model for the evolution of placentation among Reptilia, which predicts specific relationships among reproductive characteristics and thus is testable by comparative analysis among other species within the Eugongylus group of Australian skinks.
Journal ArticleDOI
Placental structure of the Australian lizard, Niveoscincus metallicus (Squamata: Scincidae)
TL;DR: A unique type of reptilian allantoplacenta was described by Weekes [1930] from a single embryonic stage of the Tasmanian skink, Niveoscincus ocellatus (as Lygosoma (Liolepisma) ocellatum) and assigned N. metallicus to this placental category but did not provide a description.
Journal ArticleDOI
Placentation in the lizard Gerrhonotus coeruleus with a comparison to the extraembryonic membranes of the oviparous Gerrhonotus multicarinatus (Sauria, Anguidae).
TL;DR: Paraffin sections of an ontogenetic series of embryos of the viviparous lizard Gerrhonotus coeruleus and the oviparious congener G. multicarinatus reveal that although general features of the development of the chorioallantoic and yolk sac membranes are similar, differences are evident in the distribution of theChorioallantois membrane in late stage embryos.
Journal ArticleDOI
Placental ontogeny of the Australian scincid lizards Niveoscincus coventryi and Pseudemoia spenceri.
TL;DR: Comparison of extraembryonic membrane and placental structure among the four viviparous and one oviparious species of Eugongylus group skinks for which data are available shows that each species exhibits characters that distinguish it from other species, while additional characters are shared among all, or some, species.
Journal ArticleDOI
Ontogeny of the extraembryonic membranes of the oviparous lizard, Eumeces fasciatus (Squamata: scincidae).
TL;DR: Oviposited eggs of Eumeces fasciatus contain embryos in the limb bud stage and one structure appears to be a site of hematopoiesis previously undescribed in vertebrates.
References
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Journal ArticleDOI
The Development of the Monotremata.—Part IV. Growth of the Ovarian Ovum, Maturation, Fertilisation, and Early Cleavage
T. Thomson Flynx,J. P. Hill +1 more
Journal ArticleDOI
Softening of hard tissue for sectioning.
R. C. Pearlman,Buell C. Cole +1 more
TL;DR: The method did not improve sectioning of tissue embedded in nitrocellulose when the blocks were stored in 70% alcohol before cutting and soaking in solutions of wetting agents (detergents) is recommended.
Journal ArticleDOI