Selective medium for isolation of Actinobacillus actinomycetemcomitans
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TLDR
With the TSBV medium, even modestly equipped laboratories will be able to isolate and identify A. actinomycetemcomitans from clinical specimens, and this bacterium from Haemophilus aphrophilus, Capnocytophaga species, and a few other contaminating organisms.Abstract:
A selective medium, TSBV (tryptic soy-serum-bacitracin-vancomycin) agar, was developed for the isolation of Actinobacillus actinomycetemcomitans, TSBV agar contained (per liter) 40 g of tryptic soy agar, 1 g of yeast extract, 100 ml of horse serum 75 mg of bacitracin, and 5 mg of vancomycin The TSBV medium suppressed most oral species and permitted significantly higher recovery of A actinomycetemcomitans than nonselective blood agar medium The distinct colonial morphology and positive catalase reaction of A actinomycetemcomitans easily distinguished this bacterium from Haemophilus aphrophilus, Capnocytophaga species, and a few other contaminating organisms With the TSBV medium, even modestly equipped laboratories will be able to isolate and identify A actinomycetemcomitans from clinical specimensread more
Citations
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Journal ArticleDOI
Actinobacillus actinomycetemcomitans in human periodontal disease
TL;DR: A large body of evidence implicates A. actinomycetemcomitans as an important micro-organism in the etiology of localized juvenile periodontitis including an increased prevalence of the organism in almost all localized juvenileperiodontitis patients and their families compared to other patient groups.
Journal ArticleDOI
Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions
TL;DR: This study demonstrated the utility of a 16S rRNA-based PCR detection method for identifying important subgingival microorganisms and indicated a strong association between the study species and periodontitis.
Journal ArticleDOI
The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing.
TL;DR: The antibacterial effect of calcium hydroxide as a short-term intracanal dressing was clinically evaluated and showed that the 7-day dressing efficiently eliminated bacteria which survived biomechanical instrumentation of the canal, while the 10-minute application was ineffective.
Journal ArticleDOI
The occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in destructive periodontal disease in adults
TL;DR: The main conclusion is that A. actinomycetemcomitans, B. gingivalis and B. intermedius are closely related to disease-active periodontitis, and more closely than to periodontal pocket depth.
Journal ArticleDOI
Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Prevalence in Patient Groups and Distribution of Biotypes and Serotypes Within Families
TL;DR: The hypothesis that A actinomycetemcomitans is an etiologic agent in this periodontal disease supported by the high prevalence in the subgingival plaque of localized juvenile periodontitis patients, compared to the much lower prevalence in other patient groups.
References
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Journal ArticleDOI
Actinobacillus actinomycetemcomitans in Human Periodontal Disease: a Cross-Sectional Microbiological Investigation
TL;DR: A. actinomycetemcomitans was on average recovered in about fivefold-higher numbers from infected deep periodontal pockets than from infected normal subgingival areas.
Journal ArticleDOI
Interaction of inflammatory cells and oral microorganisms. VIII. Detection of leukotoxic activity of a plaque-derived gram-negative microorganism.
TL;DR: The cytotoxic effect of Y4 for polymorphonuclear leukocytes presented in this study is the first report of a direct offensive microbial vector in a plaque-derived microorganism and may prove to be relevant in the pathogenesis of juvenile periodontitis.
Extraction andPartial Characterization ofaLeukotoxin from aPlaque-Derived Gram-Negative Microorganism
TL;DR: The plaque-derived gram-negative microorganism Y4 identified as a member of the genus Actinobacillus, was tested for a soluble cytotoxic factor(s) as discussed by the authors.
Journal ArticleDOI
Extraction and partial characterization of a leukotoxin from a plaque-derived Gram-negative microorganism.
TL;DR: Results suggested that a soluble leukotoxin with specificity for only human PMNs and monocytes can be liberated from viable Y4, one of the first materials from a plaque-derived microorganism with a potential role in the pathogenesis of juvenile periodontitis.
Journal ArticleDOI
Biochemical and morphological characterization of the killing of human monocytes by a leukotoxin derived from Actinobacillus actinomycetemcomitans.
TL;DR: The evidence to be presented suggests that the cell membrane of the monocyte may be the primary target in the development of juvenile periodontitis, and it is conceivable that disruption of polymorphonuclear leukocytes and monocytes by Y4 toxin in the gingival crevice area may be relevant in the pathogenesis of juvenileperiodontitis.
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