Journal ArticleDOI
Specificity of α-chymotrypsin. Separation of polar, steric, and specific effects in the α-chymotrypsin-catalyzed hydrolysis of acyl-substituted p-nitrophenyl esters
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This article is published in Biochemistry.The article was published on 1973-07-03. It has received 32 citations till now. The article focuses on the topics: Chymotrypsin & Steric effects.read more
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Kinetics of subtilisin and thiolsubtilisin.
Manfred Philipp,Myron L. Bender +1 more
TL;DR: The observation that these transition state analog inhibitors bind poorly to thiol Subtilisin while other compounds bind nearly equally well to both enzymes suggests that thiolsubtilisin may not be able to stabilize the transition state during acylation by specific substrates.
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Esterases in histochemistry and ultrahistochemistry.
O. V. Deimling,A. Böcking +1 more
TL;DR: 8-hydroxyquinoline acetate is a useful substrate in ultrahistochemistry and there is evidence of a relationship between esterase distribution and function.
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Structure‐Activity Relationships in Acetylcholinesterase Reactions
TL;DR: A fairly complete understanding of acetylcholinesterase specificity is possible after a linear relationship between the substrate non-covalent binding capacity and the leaving group hydrophobicity and a multiparameter correlation of the acetylation reaction rate constant logarithm are established.
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Juvenile hormone esterases of Lepidoptera. II: Isoelectric points and binding affinities of hemolymph juvenile hormone esterase and binding protein activities
TL;DR: Data indicate that the species currently used in the study of the developmental biology of the Lepidoptera are biochemically similar to a variety of other species in this order.
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The stereochemical resolution of enantiomeric free and derivatized amino acids using an HPLC chiral stationary phase based on immobilized alpha-chymotrypsin: chiral separation due to solute structure or enzyme activity.
TL;DR: The deactivation of the hydrolytically active site of the enzyme demonstrated that a significant portion of the retention on this support is due to hydrophobic interactions at other sites, appearing to be stereoselective for the ester derivatives of amino acids but not for the other studied solutes.
References
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Hydrogen ion buffers for biological research.
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A New Substituent Constant, π, Derived from Partition Coefficients
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Steric effects in organic chemistry
TL;DR: In this article, the authors propose that having more aspects to know and understand will lead to becoming a more precious person, and becoming more precious can be situated with the presentation of how your knowledge much.
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