Journal ArticleDOI
Spectrophotometric Determination of Cyanoglucosides in Cassava
Bala Nambisan,S. Sundaresan +1 more
TLDR
A new method is reported for determination of cyanoglucosides in cassava, simple, rapid, and sensitive, and complete CNG extraction and rapid inactivation of endogenous linamarase is possible with 80% ethanol.Abstract:
A new method is reported for determination of cyanoglucosides in cassava. The method is simple, rapid, and sensitive. Ten g cassava tuber is homogenized with warm (65-70 degrees C) 80% ethanol (1 + 6, w/v) to extract cyanoglucosides (CNG). The ethanol is evaporated, and an aliquot of the extract (0.1-0.2 mL) is incubated with added linamarase in pH 6.0 phosphate buffer for 15 min at 30 degrees C. The reaction is stopped by adding 0.2 N sodium hydroxide, the solution is neutralized, and cyanide is estimated by adding chloramine T and barbituric acid-pyridine reagent and measuring the absorbance at 570 nm. Complete CNG extraction and rapid inactivation of endogenous linamarase is possible with 80% ethanol. There is no interference from extractives in the linamarase reaction or in the estimation of cyanide. Recovery of added linamarin (as cyanide) is 98% by this assay. The minimum detection limit of cyanide in the assay is 0.1 micrograms/mL.read more
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Journal ArticleDOI
Distribution of linamarin and its metabolising enzymes in cassava tissues
Bala Nambisan,S. Sundaresan +1 more
TL;DR: High activity of β-cyanoalanine synthase in all tissues indicated that the major pathway of cyanoglucoside turnover in cassava was by conversion to asparagine.
Journal ArticleDOI
Quantitative analysis of linamarin in cassava using a cassava β-glucosidase electrode
Hock-Hin Yeoh,Van-Den Truong +1 more
TL;DR: In this paper, a cassava β-glucosidase (linamarase) electrode was used for quantifying the linamarin content of cassava roots, which ranged from 24 to 395 mg per 100 g fr wt. root.
Journal ArticleDOI
Effects of aqueous and ethanolic leaf extracts from drumstick tree (Moringa oleifera) on gilthead seabream (Sparus aurata L.) leucocytes, and their cytotoxic, antitumor, bactericidal and antioxidant activities.
José María García-Beltrán,Abdallah Tageldein Mansour,Ahmed Saud Alsaqufi,Hayssam M. Ali,María Ángeles Esteban +4 more
TL;DR: The results as a whole for the cytotoxic, bactericidal and antioxidant activities of M. oleifera leaf extracts point to their possible use as additives in functional diets for farmed fish.
Journal ArticleDOI
Rubber (Hevea brasiliensis) seed oil toxicity effect and Linamarin compound analysis.
TL;DR: No hazardous linamarin extract from RSOh, RSOchl+mth and RSOeth and LC50 were found to be (211.70 , 139.40 %, and 117.41%, respectively).
Journal ArticleDOI
Chemical composition and nutritional potential of vigna unguiculata ssp. cylindrica (fabaceae)
TL;DR: Seeds of Vigna unguiculata ssp. cylindrica (L.) Estt. (Fabaceae) were analyzed for proximate composition, proteins, fatty acids, minerals, antinutritional factors and phytohaemagglutinating activity as discussed by the authors.