Journal ArticleDOI
Sperm motility parameters for Steindachneridion parahybae based on open‐source software
Eduardo Antônio Sanches,Ronan Maciel Marcos,Renan Yoshiharu Okawara,Danilo Caneppele,Robie Allan Bombardelli,Elizabeth Romagosa +5 more
TLDR
The CASA plugin within ImageJ can be applied in sperm analysis of the study species by using the established settings and the settings used in the CASA dialogue box were satisfactory, and the results were consistent.Abstract:
Summary
The objective of this work was to evaluate the sperm motility of 13 Steindachneridion parahybae males using open-source software (ImageJ/CASA plugin). The sperm activation procedure and image capture were initiated after semen collection. Four experimental phases were defined from the videos captured of each male as follows: (i) standardization of a dialogue box generated by the CASA plugin within ImageJ; (ii) frame numbers used to perform the analysis; (iii) post-activation motility between 10 and 20 s with analysis at each 1 s; and (iv) post-activation motility between 10 and 50 s with analysis at each 10 s. The settings used in the CASA dialogue box were satisfactory, and the results were consistent. These analyses should be performed using 50 frames immediately after sperm activation because spermatozoa quickly lose their vigor. At 10 s post-activation, 89.1% motile sperm was observed with 107.2 μm s−1 curvilinear velocity, 83.6 μm s−1 average path velocity, 77.1 μm s−1 straight line velocity; 91.6% were of straightness and 77.1% of wobble. The CASA plugin within ImageJ can be applied in sperm analysis of the study species by using the established settings.read more
Citations
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Journal ArticleDOI
Aluminum, at an environmental concentration, associated with acidic pH and high water temperature, causes impairment of sperm quality in the freshwater teleost Astyanax altiparanae (Teleostei: Characidae)
João Paulo Silva Pinheiro,Cecília Bertacini de Assis,Eduardo Antônio Sanches,Renata Guimarães Moreira +3 more
TL;DR: In this article, the effects of metals, including aluminum (Al), and water temperature on ectotherms of A. altiparanae have been investigated and the individual or association effect of these variables (Al+acidic pH+temperature changes) on sperm quality has been investigated.
Journal ArticleDOI
Diets containing crude glycerin damage the sperm characteristics and modify the testis histology of Nile tilapia broodstock
Juliana Kasper Mewes,Fábio Meurer,Lucélia Tessaro,Alexandre Henrique Buzzi,Mirna Adriane Syperreck,Robie Allan Bombardelli +5 more
TL;DR: The results show that, although crude glycerin does not impair growth, any inclusion level in the diet impairs the spermatogenesis process and damages the sperm characteristics of male Nile tilapia.
Journal ArticleDOI
Lysine in the diet of Rhamdia voulezi male broodstocks confined in net cages
Odair Diemer,Fábio Bittencourt,Leonardo José Gil Barcellos,Wilson Rogério Boscolo,Aldi Feiden,Elizabeth Romagosa +5 more
TL;DR: The increment of lysine in the diet provided linear increase for weight gain and seminal volume and linear decrease for feed conversion in R. voulezi broodstocks confined in net cages.
Journal ArticleDOI
Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
Renato Massaaki Honji,Danilo Caneppele,Matías Pandolfi,Fabiana Laura Lo Nostro,Renata Guimarães Moreira +4 more
TL;DR: To identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captivity.
Journal ArticleDOI
Sperm characteristics of Steindachneridion parahybae (Steindachner, 1877) throughout 112 h of storage at four temperatures
Eduardo Antônio Sanches,Renan Yoshiharu Okawara,Danilo Caneppele,Cesar Pereira Rebechi de Toledo,Robie Allan Bombardelli,Elizabeth Romagosa +5 more
TL;DR: It is suggested that short-term semen storage – if necessary – should be within a range of 15–25°C, and Pearson's linear correlation was verified between the parameters studied, indicating MOT, VCL and STR as good indicators of sperm quality.
References
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Journal ArticleDOI
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Journal ArticleDOI
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