Journal ArticleDOI
Staining Pollen Tubes in the Style; Cotton Blue versus Carmine for General Use
P. C. Datta,Anita Naug +1 more
TLDR
This procedure was found to be more generally applicable to angiosperms than carmine, applied in propionic-lactic acid mixtures.Abstract:
Pollen tubes passing through the style are fixed in acetic-alcohol (1 : 1) at 60 C for 30 min; softened by boiling in water 5–30 min; cleared in lactic acid at 60 C, 30 min; stained in lactophenol-cotton blue (1%) at 60 C, 15–30 min; and mounted in lactic acid. This procedure was found to be more generally applicable to angiosperms than carmine, applied in propionic-lactic acid mixtures. It has been tested on 36 species, including 4 monocotyledons and 32 dicotyledons.read more
Citations
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Sensitivity of forest plant reproduction to long range transported air pollutants: in vitro and in vivo sensitivity of oenothera parviflora l. pollen to simulated acid rain
TL;DR: The response of pollen in vitro indicated significant inhibitory effects of pH, and demonstrated that pH values ≤ 3.6 were inhibitory to both germination and tube growth, when compared with the treatment of pH 5.6.
Journal ArticleDOI
Pollination of kiwifruit (Actinidia chinensis Planch.): Stigma-style structure and pollen tube growth
TL;DR: The structure of stigma — style tissues in kiwifruit flowers was examined before, and after, hand-pollination and the course of pollen tube development was followed.
Journal ArticleDOI
Genetics of the incompatibility system in the crucifer Eruca sativa L
TL;DR: This communication is intended to present the data, particularly of F2 and F3 families worked out by Miss Indra Dhir and Miss Renuka Malik respectively, and to interpret the same regarding the nature of the incompatibility system.
Journal ArticleDOI
Protection of pollen germination from adverse temperatures: a possible role for proline
Zhang Hong-qi,Anton F. Croes +1 more
TL;DR: The results suggest that the high proline concentrations found in pollen of many species may confer resistance to germinating pollen grains at unfavourable temperatures thereby enhancing the chances of successful fertilization.
Journal ArticleDOI
Staining pollen tubes in the styles of cereals with cotton blue; fixation by ethanol-lactic acid for enhanced differentiation.
TL;DR: In this paper, the authors used Pollen Tubes in the Styles of Cereals with Cotton Blue; Fixation by Ethanol-Lactic acid for Enhanced Differentiation, which is used for stain technology.
References
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Hybridization of maize, tripsacum, and euchlaena
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Pollen-Tube Growth of Ten Species of Datura in Interspecific Pollinations.
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