Journal ArticleDOI
Stoichiometric relation of protein components in cerebral myelin from different species.
TLDR
Using the polyacrylamide‐gel electrophoresis it was possible to demonstrate that a homogeneous structural protein constitutes about 50 percent of the total amount of myelin proteins in all species studied.Abstract:
— In cerebral myelin from man, ox, rabbit, guinea pig and chicken, the amounts of proteolipid protein, basic protein and the fraction of further protein components were found to be present in a fixed ratio of 5·0: 3·5: 2·0 by weight. The molecular weights of 25,000 and 35,000 as obtained for the basic protein and proteolipid protein might indicate that cerebral myelin contains one molecule of basic protein per molecule of proteolipid protein. This fixed ratio of protein components was found to be changed in myelin from the PNS and in cerebral myelin from rat and carp, with their exceptional basic proteins. Using the polyacrylamide-gel electrophoresis it was possible to demonstrate that a homogeneous structural protein (the Folch-Lees proteolipid protein) constitutes about 50 percent of the total amount of myelin proteins in all species studied. An attempt was made to correlate myelin protein and lipid patterns from various species.read more
Citations
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Book ChapterDOI
Isolation and Characterization of Myelin
TL;DR: This chapter will discuss isolation procedures and the composition of purified myelin obtained by these procedures, and the techniques developed for isolating myelin membranes essentially free of other subcellular structures.
Journal ArticleDOI
Protein composition of myelin of the peripheral nervous system
TL;DR: The results indicated that the P1 protein is very similar in size and amino acid composition to the basic protein of central nervous system myelin, whereas the P0 and P2 proteins are unique to the PNS.
Journal ArticleDOI
Quaking mouse: isolation and characterization of myelin protein
TL;DR: A new technique, involving final purification on a continuous CsCl gradient, was utilized for the isolation of cerebral myelin from adult quaking mice, littermate controls and young (10‐day‐old) normal mice.
Journal ArticleDOI
Evidence for the close association of a glycoprotein with myelin in rat brain.
TL;DR: The results obtained from these experiments suggested that the glycoprotein is closely associated with myelin and that it is not in an unrelated contaminating structure.
Journal ArticleDOI
Changes in the protein composition of mouse brain myelin during development.
TL;DR: Myelin protein accumulated throughout development, increasing from 0.25 mg of protein/brain at 8 days of postnatal age to 3.5 mg at 300 days, although the rate of accumulation was greatest at about 21 days of age, according to a hypothesis relating the observed changes in protein composition of myelin during development to its mode of formation.
References
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Journal ArticleDOI
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
Jordi Folch,Marjorie B. Lees +1 more
Journal ArticleDOI
Distribution of lipids in subcellular particles of guinea-pig brain.
TL;DR: Detailed analyses of the individual lipids present in such subfractions are described by using a recently developed method for the determination of all known phospholipids (Dawson, Hemington & Davenport, 1962); it provides new data on the lipid composition of brain mitochondria, nuclei and microsomes, as well as the subcellular particles peculiar to brain: myelin fragments, synaptosomes and synaptic vesicles.
Journal ArticleDOI
The lipid composition of purified bovine brain myelin
TL;DR: The detailed analysis of both fractions obtained were called light and heavy, according to their relative positions after centrifugation in a sucrose density gradient, and the lipid composition of the light myelin fractions has already been reported.