STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells
TLDR
Normal and transformed baby hamster kidncy (BHK) cells attach to Falcon polystyrene with the same first order rate constant and recovery of adhesiveness of trypsin-trcated cells is inhibitcd by cycloheximidc.Abstract:
Normal and transformed baby hamster kidncy (BHK) cells attach to Falcon polystyrene with the same first order rate constant. The longer the cclls arc attached to the bottlcs, thc more difficult they are to rcmovc. Sulfhydryl (--SH) binding rcagcnts inhibit both the attachment of BHK cells and the increase in adhesive strength of attached cells. Attached BHK cells bind fewer molecules of [1-14C]N-cthylamleimide (an --SH binding reagent) than do suspendcd cells. Incubation of cells with high concentrations of trypsin results in a reversible loss of cell adhcsivcness. Thc recovery of adhesiveness of trypsin-trcated cells is inhibitcd by cycloheximidc.read more
Citations
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Journal ArticleDOI
Bacterial adhesion and biofilms on surfaces
TL;DR: An overview ofacterial adhesion is provided, examples of how bacterial adhesion affects industry are cited, and methods and instrumentation used to improve the understanding of the adhesive properties of bacteria are summarised.
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Cellular adhesiveness and extracellular substrata.
TL;DR: Adhesiveness is common to many different cell types under a variety of conditions, and the underlying mechanisms are analogous for active cell-substratum adhesion occurring in vitro to artificial and to model physiological substrata and in vivo to fibr inogen or fibrin deposits.
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Fibronectin: a review of its structure and biological activity.
TL;DR: Fibronectin (Fn) is a widely accepted designation for both plasma and cell associated forms of this protein and will be used throughout this review to represent both states of the protein.
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A scanning electron microscope study of cell adhesion and spreading in vitro.
TL;DR: Using normal diploid WI-38 cells, the changes in cell morphology during adhesion and flattening on to the glass surface in vitro were studied with a scanning electron microscope to find a probable mechanism responsible for the morphological events during cell adhesion.
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The interaction of plasma fibronectin with fibroblastic cells in suspension.
TL;DR: The binding parameters determined in this study further define the fibroblast cell-surface fibronectin receptor, which is resistant to trypsin in the presence of physiological concentrations of divalent cations but is susceptible to tryPSin inThe presence of 5 mM EDTA.
References
More filters
Journal ArticleDOI
Metabolic coupling, ionic coupling and cell contacts.
TL;DR: Observations using electron microscopy and freeze-etching techniques show that both metabolic coupling and ionic coupling in fibroblasts are associated with the appearance of “gap” junctions.
Journal Article
The surface properties of cancer cells: a review.
M Abercrombie,E J Ambrose +1 more
TL;DR: In recent years attention has been increasingly turning to the cell surface as the seat of an impor tant part of the malignant transformation, and this crucial aspect of the cancer problem must still be the main preoccupation of a review on the cellsurface in relation to cancer.
Journal ArticleDOI
The physical chemistry of cell adhesion.
TL;DR: Calculations of repulsion barriers to adhesion between approaching cells indicate that villi and other small membrane protuberances are the most likely regions of initial contact.
Journal ArticleDOI
Attachment and spreading of cells in culture
TL;DR: The attachment and spreading of cells settled from suspension as affected by the nature of the substratum and the composition of the medium were studied.