Study on transdermal absorption characteristics of brucine in vitro

TLDR: The research provides experimental datas for the design of transdermal delivery system of brucine in vitro and provides experimental data on drug permeation tests performed through excised rat skin in improved Franz diffusion cell.

Abstract: OBJECTIVE To study the transdermal absorption characteristics of brucine in vitro. METHODS The oil-water partition coefficient of brucine was measured. Drug permeation tests were performed through excised rat skin in improved Franz diffusion cell. Brucine concentration in samples was determined by HPLC. RESULTS The oil-water partition coefficient of brucine was between 4.05 and 5.02 at different temperatures. The permeation rate of 0.5 and 1 mg/ml brucine solution were (1.83 +/- 0.85) and (3.74 +/- 1.54) microg/cm2 x h, respectively. The cumulative permeation ratio in 24 hours were (51.30 +/- 18.51)% and (50.01 +/- 12. 80)%, respectively. CONCLUSION The research provides experimental datas for the design of transdermal delivery system of brucine.