Journal ArticleDOI
Temperature sensitivity of human spermatogonia and spermatocytes in vitro.
Masahide Nakamura,Mikio Namiki,Akihiko Okuyama,T Matsui,Y Doi,Masami Takeyama,Hideki Fujioka,Yoshitake Nishimune,K Matsumoto,Takao Sonoda +9 more
TLDR
It seems that in normal body temperature (37 degrees C) differentiated germ cells such as spermatids and spermatozoa are fragile and the DNA synthesis of sper matogonia and resting primary sperMatocytes is retarded.Abstract:
To study the effect of temperature on human spermatogenesis, both the number and DNA synthesis of germ cells were investigated in tissue fragments of human testes cultured for 22 h at 31 degrees C and 37 degrees C. The number of differentiated germ cells such as spermatids and spermatozoa cultured at 37 degrees C was significantly smaller than that cultured at 31 degrees C. The number of spermatogonia and resting primary spermatocytes was not significantly different between these two temperatures, but the functional ability of DNA synthesis in these cells was significantly lower at 37 degrees C than at 31 degrees C. It seems that in normal body temperature (37 degrees C) differentiated germ cells such as spermatids and spermatozoa are fragile and the DNA synthesis of spermatogonia and resting primary spermatocytes is retarded.read more
Citations
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Journal ArticleDOI
The Parkes Lecture. Heat and the testis
TL;DR: The evidence for the lower temperature of the testes of many mammals is summarized, and the reasons suggested for the descent of thetestes into a scrotum are discussed.
Journal ArticleDOI
Varicocele and male infertility: Part II: Pathophysiology of varicoceles in male infertility
TL;DR: The role of hyperthermia, testicular blood flow and venous pressure changes, reflux of renal/adrenal products, hormonal dysfunction, autoimmunity, defects in acrosome reaction, and oxidative stress, in the pathophysiology of varicocele will be discussed.
Journal ArticleDOI
Biochemical Changes in Testicular Varicocele
TL;DR: This overview includes the available information on the biochemical change in the testes associated with varicocele as well as the introduction of basic biochemical aspects on the testis, which may give new insights into the possible pathophysiological mechanism of male infertility.
Journal ArticleDOI
Establishment and in vitro culture of porcine spermatogonial germ cells in low temperature culture conditions
Won-Young Lee,Hyun-Jung Park,Ran Lee,Kyung Hoon Lee,Yong-Hee Kim,Buom-Yong Ryu,Nam-Hyung Kim,Jin-Hoi Kim,Jae-Hwan Kim,Sung-Hwan Moon,Jin-Ki Park,Hak-Jae Chung,Dong-Hoon Kim,Hyuk Song +13 more
TL;DR: In this paper, a porcine spermatogonial germ cell (pSGC) line was established and cultured for long periods under a low temperature culture environment in vitro, and pSGCs could stably maintain undifferentiated germ cell and stem cell characteristics for over 60 days during culture at 31°C.
Journal ArticleDOI
Is the laparoscopic Palomo procedure for pediatric varicocele safe and effective? Nine years of unicentric experience.
A. Pini Prato,G. A. MacKinlay +1 more
TL;DR: The laparoscopic Palomo procedure is a safe and effective surgical option for the treatment of pediatric varicocele, although it carries a fairly high risk of postoperative hydrocele.
References
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Journal ArticleDOI
Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.
Journal ArticleDOI
Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males.
TL;DR: A new and rapid method for registration of spermatogenesis in human testes: the testicular biopsy score count is described.
Journal ArticleDOI
Mammalian testes in organ culture.
TL;DR: Tests of 14-day-old rats were grown as organ cultures for periods of up to 6 months and a cell type, resembling primitive germ cells of younger animals, consistently appeared in seminiferous tubules on the fourth day of culture.
Journal ArticleDOI
Quantitation of the cells of the seminiferous epithelium of the human testis employing the Sertoli cell as a constant
Mavis J. Rowley,Carl G. Heller +1 more
TL;DR: Two variants of a method for determining the quantitative structure of the human seminiferous epithelium are described and quantitation of biopsies from one individual before and after testicular X-ray irradiation show how the method can be applied.
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