Tetrazolium chloride as a test reagent for freezing injury of seed corn
Norah Bennett,W. E. Loomis +1 more
TLDR
Staining methods involving the reduction of selenium salts or of tetrazolium compounds to colored forms by the action of living cells seem to be the most promising quick tests of viability for estimating viability in seeds.Abstract:
Rapid methods for estimating viability in seeds have been suggested by a number of workers. Most of these are dependent upon the increased permeability (6, 10) or decreased reducing power (5, 8) of dead cells or tissues. Staining methods involving the reduction of selenium salts (4) or of tetrazolium compounds (9, 11) to colored forms by the action of living cells seem to be the most promising quick tests of viability. These tests should be particularly applicable to estimations of freezing injury in seed corn because: (a) corn is easily tested (11), (b) immature corn shows a degree of dormancy (13) which makes routine laboratory tests of germinability difficult and slow, and (c) speed is always essential to determine whether frozen seed corn is sufficiently viable to justify expensive processing, or whether the crop should be diverted to other uses.read more
Citations
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Studies on the reduction of 2,3,5-triphenyltetrazolium chloride as a viability assay for plant tissue cultures
Leigh E. Towill,Peter Mazur +1 more
TL;DR: Optimum conditions for the reduction of 2,3,5-triphenyltetrazolium chloride to a water-insoluble red formazan have been determined for suspension tissue cultures of Acer saccharum and Haplopa.
Journal ArticleDOI
Interactions between litter and water availability affect seedling emergence in four familial pairs of floodplain species
TL;DR: The results suggest that transfer of seed-containing plant litter can aid restoration projects if applied at 0.2–0.4 kg m−2, and the sign of the litter effect on seedling emergence depends on soil humidity, which is species- and family-specific and is closely related to seed size.
Journal ArticleDOI
Heat Shock Protein Synthesis and Thermal Tolerance in Wheat
TL;DR: A correlation between the synthesis of specific low molecular weight heat shock proteins and the degree of thermal tolerance expressed following exposure to elevated temperatures is provided.
Book ChapterDOI
The Evaluation of Crop Plants for Winter Hardiness
TL;DR: In this article, the authors present several methods that have seemed to have promise, and numerous physiological studies have considerably advanced the knowledge of winter hardiness and the hardening process, and many of these methods have been applied or appraised adequately by agronomists; an excellent opportunity exists for the development of new and better methods for the evaluation of the winter hardness of crop plants.
References
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Journal ArticleDOI
Investigations of the hardiness of plants by measurement of electrical conductivity
TL;DR: Most, if not all, of the theories of winter-hardiness are built around the idea of the water-relations of the plant, and that structural, osmotic or colloidal properties of plants are shown to be associated with hardiness.
Journal ArticleDOI
The Use of 2,3,5-Triphenyl-Tetrazoliumchloride as a Measure of Seed Germinability
TL;DR: Data is published indicating that the determination of the catalase ratio of dry and gernniilatiing seeds may serve as a measure of seed viability and the anmylase activity of soaked oat seed is definitely correlated with geriminability.
Journal ArticleDOI
The Relation of Moisture Content and Time of Harvest to Germination of Immature Corn1
TL;DR: It is suggested that the mechanism which inhibits the normal germination of newly harvested immature corn operates in the scutellum rather than in the endosperm or pericarp.