The aminopeptidase from Aeromonas proteolytica: structure and mechanism of co-catalytic metal centers involved in peptide hydrolysis
TLDR
The aminopeptidase from Aeromonas proteolytica (AAP) is one of the best mechanistically characterized hydrolytic enzymes that contains a dinuclear center and is, therefore, the focus of this review.About:
This article is published in Coordination Chemistry Reviews.The article was published on 2002-10-01 and is currently open access. It has received 75 citations till now. The article focuses on the topics: Protein degradation & Aminopeptidase.read more
Figures
Fig. 1. Ribbon diagram of the X-ray crystal structure of AAP based on the coordinates from the Protein Data Bank (1AMP). The two zinc(II) ions are depicted as green spheres. ![Fig. 3. Drawing of the active site of [ZnZn(AAP)] along with the proposed active site geometry of [CoCo(AAP)] based on spectroscopic studies.](/figures/fig-3-drawing-of-the-active-site-of-znzn-aap-along-with-the-1siartmu.png)
Fig. 3. Drawing of the active site of [ZnZn(AAP)] along with the proposed active site geometry of [CoCo(AAP)] based on spectroscopic studies. 
Table 2. Summary of the inhibition constants for aliphatic alcohol inhibition of AAP at pH 8.0 ![Fig. 13. Drawings of the active sites of [ZnZn(AAP)] and [ZnZn(CPG2)] based on X-ray crystallography.](/figures/fig-13-drawings-of-the-active-sites-of-znzn-aap-and-znzn-qoht5lev.png)
Fig. 13. Drawings of the active sites of [ZnZn(AAP)] and [ZnZn(CPG2)] based on X-ray crystallography. 
Fig. 10. Proposed mechanism of peptide hydrolysis catalyzed by AAP. Protein ligands to the metal ions are shown only in the first panel in order to avoid confusion. ![Fig. 4. Electronic absorption spectra of [CoCo(AAP)], [Co_(AAP)], [CoZn(AAP)], and [ZnCo(AAP)] in 50 mM HEPES buffer at pH 7.5 containing 150 mM KCl.](/figures/fig-4-electronic-absorption-spectra-of-coco-aap-co-aap-cozn-3rm5a0zh.png)
Fig. 4. Electronic absorption spectra of [CoCo(AAP)], [Co_(AAP)], [CoZn(AAP)], and [ZnCo(AAP)] in 50 mM HEPES buffer at pH 7.5 containing 150 mM KCl.
![Fig. 3. Drawing of the active site of [ZnZn(AAP)] along with the proposed active site geometry of [CoCo(AAP)] based on spectroscopic studies.](/figures/fig-3-drawing-of-the-active-site-of-znzn-aap-along-with-the-1siartmu.webp)
![Fig. 13. Drawings of the active sites of [ZnZn(AAP)] and [ZnZn(CPG2)] based on X-ray crystallography.](/figures/fig-13-drawings-of-the-active-sites-of-znzn-aap-and-znzn-qoht5lev.webp)
![Fig. 4. Electronic absorption spectra of [CoCo(AAP)], [Co_(AAP)], [CoZn(AAP)], and [ZnCo(AAP)] in 50 mM HEPES buffer at pH 7.5 containing 150 mM KCl.](/figures/fig-4-electronic-absorption-spectra-of-coco-aap-co-aap-cozn-3rm5a0zh.webp)
Citations
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Mode of action of bi- and trinuclear zinc hydrolases and their synthetic analogues.
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Crystal structure of prostate-specific membrane antigen, a tumor marker and peptidase
TL;DR: The 3.5-Å crystal structure of the PSMA ectodomain is presented, which reveals a homodimer with structural similarity to transferrin receptor, a receptor for iron-loaded transferrin that lacks protease activity.
Journal ArticleDOI
Zinc hydrolases: the mechanisms of zinc-dependent deacetylases.
Marcy Hernick,Carol A. Fierke +1 more
TL;DR: This review focuses on the current understanding of the mechanisms catalyzing by various zinc-dependent deacetylases and, in particular, the reaction mechanism catalyzed by the enzyme UDP-3-O-(R- 3-hydroxymyristoyl)-N-acetylglucosamine deacetyase, also known as LpxC.
Journal ArticleDOI
Effect of Zn...Zn separation on the hydrolytic activity of model dizinc phosphodiesterases.
TL;DR: Comparative reactivity studies on the cleavage of bis(4-nitrophenyl)phosphate mediated by the two dizinc complexes have revealed that the system with the larger Zn...Zn separation is hydrolytically more potent, both in the hydrolysis and the transesterification of BNPP.
Journal ArticleDOI
Crystal structures of human glutaminyl cyclase, an enzyme responsible for protein N-terminal pyroglutamate formation
TL;DR: The structure of human QC in free form and bound to a substrate and three imidazole-derived inhibitors is presented and a catalysis mechanism of the formation of protein N-terminal pGlu is proposed.
References
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Book
Advanced Inorganic Chemistry
TL;DR: Cotton and Wilkinson's Advanced Inorganic Chemistry (AIC) as discussed by the authors is one of the most widely used inorganic chemistry books and has been used for more than a quarter century.
Book
Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems
TL;DR: Kinetics of Unireactant Enzymes.
Journal ArticleDOI
Structural and Functional Aspects of Metal Sites in Biology
TL;DR: The authors present here a classification and structure/function analysis of native metal sites based on these functions, and the coordination chemistry of metalloprotein sites and the unique properties of a protein as a ligand are briefly summarized.
Journal ArticleDOI
Zinc coordination, function, and structure of zinc enzymes and other proteins
Bert L. Vallee,David S. Auld +1 more
Journal ArticleDOI
Recent Advances in Zinc Enzymology
TL;DR: Zinc enzymology is, compared to some other current areas of metallobiochemistry, a maturing field, but in addition to further developments of structure-function relationships it has also provided a number of surprising new results and ideas in the last few years.