Journal ArticleDOI
The effect of drugs on the labeling of blood elements with technetium-99m.
A. C.S. Braga,Márcia Betânia Nunes de Oliveira,Gláucio Diré Feliciano,Ingrid Wanke Reiniger,J. F. Oliveira,C.R Silva,Mario Bernardo-Filho +6 more
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TLDR
This study shows that extracts of some medicinal plants can affect the radiolabeling of red blood cells with 99mTc using an in vitro technique.Abstract:
The influence of drugs on the labeling of red blood cells and plasma proteins with 99mTc has been reported. Any drug, which alters the labeling of the tracer, could be expected to modify the disposition of the radiopharmaceuticals. Red blood cells (RBC) labeled with technetium-99m (99mTc) are used for several evaluations in nuclear medicine. We have evaluated the effect of Thuya occidentalis, Peumus boldus and Nicotiana tabacum (tobacco) extracts on the labeling of RBC and plasma and cellular proteins with 99mTc. Blood was incubated with the drugs. Stannous chloride (SnCl2) solutions and 99mTc were added. Plasma (P) and blood cells (BC) were separated. The percentage of radioactivity (%ATI) bound to P and BC was determined. The %ATI on the plasma and cellular proteins was also evaluated by precipitation of P and BC samples with trichloroacetic acid (TCA) and isolation of soluble (SF) and insoluble (IF) fractions. The analysis of the results shows that there is a decrease in %ATI (from 97.64 to 75.89 percent) in BC with Thuya occidentalis extract. The labeling of RBC and plasma proteins can be decreased in presence of tobacco. This can be due either a direct or indirect effect (reactive oxygen species) of tobacco. The analysis of radioactivity in samples of P and BC isolated from samples of whole blood treated with Peumus boldus showed a rapid uptake of the radioactivity by blood cells in the presence of the Peumus boldus, whereas there was a slight decrease in the amount of 99mTc radioactivity in the TCA-insoluble fraction of plasma. This study shows that extracts of some medicinal plants can affect the radiolabeling of red blood cells with 99mTc using an in vitro technique.read more
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Journal ArticleDOI
Drug interaction with radiopharmaceuticals: a review
Mario Bernardo-Filho,Sebastião David Santos-Filho,Egberto Gaspar de Moura,Adalgisa Ieda Maiworm,Margarida Maria de Camões Orlando,Maria Expósito Penas,Valbert Nascimento Cardoso,Luciana Camargo Bernardo,Lavínia C. Brito +8 more
TL;DR: Factors capable of interfering with the bioavailability of the radiobiocomplexes is worthwhile for secure diagnosis and the development of biological models to study these phenomena is highly relevant and desirable.
Journal ArticleDOI
Effect of extract of medicinal plants on the labeling of blood elements with Technetium-99m and on the morphology of red blood cells: I—a study with Paullinia cupana
J.F de Oliveira,A.S Ávila,A. C.S. Braga,M. B. N. de Oliveira,Edson Boasquevisque,R.L. Jales .,Valbert Nascimento Cardoso,Mario Bernardo-Filho +7 more
TL;DR: In this article, Paullinia cupana is used in popular medicine and the authors evaluated its influence on the labeling process using technetium-99m (Tc99m).
Journal ArticleDOI
Assessment of the effect of Fucus vesiculosus extract on the labeling of blood constituents with technetium-99m and the histological modifications on the shape of the red blood cells.
J Fonseca de Oliveira,M.B Nunes de Oliveira,A.S Ávila,A. C.S. Braga,M.T Jansen de Almeida Catanho,R. L. Cavalcanti Jales,Valbert Nascimento Cardoso,M Bernado-Filho +7 more
TL;DR: Investigation of the possibility that F. vesiculosus extract is capable of altering the labeling of blood elements with 99mTc revealed that this extract is able of promoting important modifications on the shape of the RBC, which would decrease the%ATI on blood elements and indicate the presence of oxidant agents in the F.
Journal ArticleDOI
Gastrointestinal Bleeding Scintigraphy in the Early 21st Century
TL;DR: The history of the techniques, the different methods of radiolabeling erythrocytes, the procedure, useful indications, diagnostic accuracy, the use of SPECT/CT and CT angiography to evaluate gastrointestinal bleeding, and Meckel diverticulum imaging are reviewed.
Journal ArticleDOI
Drug interaction with radiopharmaceuticals: effect on the labeling of red blood cells with technetium-99m and on the bioavailability of radiopharmaceuticals
TL;DR: The development of biological models to study the DIR is highly relevant because the consequences are the possibility of misdiagnosis and/or the repetition of the examination with an increase of radiation dose to the patient.