Journal ArticleDOI
The effect of pH on staining by eosins.
TLDR
Variations in pH elicited different degrees of stain retention; different fixation altered staining affinity even though staining was effected at identical pH values.Abstract:
The effect of pH on staining by 7 dyes of the eosin group was studied by means of specimens fixed 12-18 hr in Gilson's, in Petrunkewitsch's, or in Zenker's fluids, respectively. Sections were soaked in water or alcohol, depending upon the stain solvent, adjusted in 31 equal steps from pH 4.0 to 10.0 before being stained in similarly adjusted dye solutions. The stained sections were rinsed at H-ion concentrations identical to that at which staining took place, until no further color came away, and mounted. Variations in pH elicited different degrees of stain retention; different fixation altered staining affinity even though staining was effected at identical pH values.read more
Citations
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Journal ArticleDOI
Counting blood cells of chickens
L. G. Chubb,J. G. Rowell +1 more
TL;DR: Although blood counts varied only slightly during the day, there were considerable differences from day to day and there were also considerable differences in blood counts between chickens even though environmental and genetic conditions were standardized as far as possible.
Journal ArticleDOI
Eosin staining of gelatine.
TL;DR: Comparing the pH dependence of dyebinding for eosin y and esterified eosins with ionization constants revealed that ionic bonding is unlikely to occur at the carboxyl group as well as at the phenolic group, and dye binding is intimately related to the presence of Br-groups.
Journal ArticleDOI
An evaluation of the metachromasy of anionic dyes. I. Visual observations on tissue sections.
TL;DR: Nuclei, erythrocytes, inner elastic laminae of arteries, keratinous structures, and certain areas in the ground substance of connective tissue most often elicited metachromasy, and it is suggested that basic proteins are responsible for the meetachromatic reactions.
References
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Journal ArticleDOI
Die Lage des isoelektrischen Punktes histologischer Elemente als Ursache ihrer verschiedenen Frbbarkeit: Ausgefhrt mit Untersttzung der Rockefeller-Foundation
TL;DR: Es wird zunachst der Einflus, den eine Anderung der CH bei der Adsorption des basischen Toluidinblaues and des sauren Cyanols durch Gelatine, Huhnereiweis, Thymus and Knorpel ausubt, untersucht.