Journal ArticleDOI
The Effect of Tapering the Optical Fiber on Evanescent Wave Measurements
TLDR
In this paper, the effect of tapering on the sensing region of the optical fibers was investigated for remote evanescent wave detection with optical fibers, where the majority of the fiber remains cladded while only a small portion of the core is exposed to perform the analysis.Abstract:
Optical biosensors are analytical tools employed in environmental, biotechnology and clinical fields. For remote evanescent wave detection with optical fibers, the majority of the fiber remains cladded while only a small portion of the core is exposed to perform the analysis. To improve the sensitivity of this type of fiber optic biosensor, tapering the sensing region of the fiber was investigated. Silica fibers were tapered into two geometries: step and continuous tapers. To determine the effect of tapering on detection levels, a fluoroimmunoassay was performed on fibers with nontapered, step-tapered and continuously tapered geometries. Solutions containing a fluorescent analyte were circulated over an antibody-coated fiber and the fluoresence signal measured The minimum detection limit for fibers with 10 cm sensing region was 1.56 nM, 0.31 nM and 0.16 nM for nontapered, step-tapered and continuously tapered fibers, respectively. Continuous tapering of the sensing region of an optical fiber offe...read more
Citations
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Journal ArticleDOI
Array biosensor for detection of biohazards
Chris A. Rowe-Taitt,Joel P. Golden,Mark J. Feldstein,John J. Cras,Karen E. Hoffman,Frances S. Ligler +5 more
TL;DR: A fluorescence-based biosensor for simultaneous analysis of multiple samples for multiple biohazardous agents and a fluidics and imaging system has been developed which allows automated detection of staphylococcal enterotoxin B in the low ng/ml range.
Journal ArticleDOI
Fibre-optic evanescent field absorption sensor based on a U-shaped probe
TL;DR: In this article, a fiber-optic evanescent field absorption sensor based on a U-shaped sensing probe is described and the influences of fiber core diameter, bending radius of the probe and the refractive index of the fluid on the sensitivity of the sensor are evaluated experimentally.
Journal ArticleDOI
Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor.
TL;DR: A rapid, sensitive, analytical method for the detection of Clostridium botulinum toxin has been developed that could be detected within a minute, at concentrations as low as 5 ng/ml and the reaction was highly specific and no response was observed against tetanus toxin.
Journal ArticleDOI
Antibody immobilization using heterobifunctional crosslinkers.
Lisa C. Shriver-Lake,Brian L. Donner,Rebecca Edelstein,Kristen Breslin,Suresh K. Bhatia,Frances S. Ligler +5 more
TL;DR: Several heterobifunctional crosslinkers are compared to GMBS for their ability to immobilize active antibodies onto glass cover slips at a high density and bound more antigen than GMBS immobilized antibodies as indicated by the fluorescence signal.
Journal ArticleDOI
A fiber optic biosensor: combination tapered fibers designed for improved signal acquisition
TL;DR: In this article, a combination taper fiber was proposed to provide an even signal return along the length of the fiber's length, where the fiber tapers down to the V-number matching radius over 1 cm, maintaining total internal reflection, then continues to taper gently along the following 9 cm to near the distal end.
References
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Book
Optical Fiber Communications
TL;DR: The concept of WDM combined with optical amplifiers has resulted in communication links that allow rapid communications between users in countries all over the world as discussed by the authors, which is known as wavelength division multiplexing (WDM).
Journal ArticleDOI
Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces.
Suresh K. Bhatia,Lisa C. Shriver-Lake,Kimberly J. Prior,Jacque H. Georger,Jeffrey M. Calvert,Reinhard Bredehorst,Frances S. Ligler +6 more
TL;DR: A procedure for covalent immobilization of functional proteins on silica substrates was developed using thiol-terminal silanes and heterobifunctional cross-linkers and is straightforward, easy to perform, and adaptable for modifying mass quantities of biosensor components.
Patent
Immobilization of active agents on substrates with a silane and heterobifunctional crosslinking agent
Frances S. Eigler,J. H. Georger,Suresh K. Bhatia,Jeff Calvert,Lisa C. Shriver-Lake,Reinhard Bredehorst +5 more
TL;DR: In this article, a silane is bound to the substrate and coupled to a heterobifunctional crosslinker at one functional group leaving a free functional group, different than the first group, to which a protein is bound while retaining high protein functionality.
Patent
Waveguide-binding sensor for use with assays
TL;DR: In this article, the authors proposed an optical waveguide-binding sensor which increases sensor sensitivity to fluorescence detection during assays of liquids based on adjustments in the V number along the waveguide.
Book ChapterDOI
Chemistry and Technology of Evanescent Wave Biosensors
TL;DR: The basic optical properties of fibers, the configurations of fiberoptic biosensors, the means of attaching biomolecules to optical surfaces, advances in fiberoptics, and related fields of interest are described.