The formation of a long-lived complex between an ATP-dependent deoxyribonuclease and DNA.
F.G. Winder,P.A. Sastry +1 more
TLDR
The results of some investigations with the M. smegmatis enzyme are reported, designed to determine whether a complex between it and the DNA could be demonstrated and whether ATP plays a role in the formation of such a complex.About:
This article is published in FEBS Letters.The article was published on 1971-09-15 and is currently open access. It has received 12 citations till now. The article focuses on the topics: Deoxyribonuclease & DNA.read more
Citations
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Book ChapterDOI
The Physiology of the Mycobacteria
TL;DR: The chapter discusses physiology to cover not only the mycobacterial cell, its structure and organization, but also the properties that the organisms must have—first to enable them to survive once having gained entry to a host, and secondly to bring about the various changes that are subsequently manifested in the host.
Book ChapterDOI
13 recBC-like Enzymes: Exonuclease V Deoxyribonucleases
TL;DR: Mechanism of action of the exoV enzyme indicates that DNA unwinding is fundamental to several of the enzyme's activities and can be its most important function in the cell.
Journal ArticleDOI
Properties of an ATP-dependent deoxyribonuclease from Micrococcus luteus Evidence for a stable DNA-enzyme complex
TL;DR: Results from kinetic experiments suggest that the formation of the enzyme-DNA complex is a necessary intermediate in the overall degradation process, its rate being proportional to the concentration of the complex.
Journal ArticleDOI
Binding of the ATP-dependent DNase from Hemophilus influenzae to duplex DNA molecules.
K W Wilcox,H O Smith +1 more
TL;DR: Using a membrane filter assay, the binding reaction between the ATP-dependent DNase from Hemophilus influenzae and duplex DNA under conditions where the degradative reaction is not allowed to proceed is studied.
Journal ArticleDOI
Role of ATP in ATP-dependent Deoxyribonuclease Activity
TL;DR: ATP-DEPENDENT deoxyribonucleases with an exonucleolytic action on native DNA have been reported from several bacterial sources, but evidence as to the role of ATP in their action is scanty, although ATP is required for the formation of a complex between the enzyme and DNA.
References
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Journal ArticleDOI
Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations.
TL;DR: The suggestion is made that restoration of recombination by indirect suppression involves an activation or derepression of one or a series of enzymes, which participate in a pathway of recombinations, alternative to the recB and recC pathway, but normally of minor importance.
Journal ArticleDOI
Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes.
TL;DR: An adenosine triphosphate-dependent deoxyribonuclease activity has been detected in lysates of recB(+)recC(+) strains, and mutations in recB or recC lead to loss of this activity, suggesting that these two genes determine the nucleaseActivity.
Journal ArticleDOI
AN ATP-DEPENDENT DEOXYRIBONUCLEASE FROM Escherichia coli WITH A POSSIBLE ROLE IN GENETIC RECOMBINATION
TL;DR: A DNase which requires ATP for its function was purified from E. coli and some of the characteristics of the purified enzyme were studied and the possible role of the enzyme in genetic recombination is discussed.
Journal ArticleDOI
Enzymatic DNA degradation in E. coli: its relationship to synthetic processes at the chromosome level.
Gérard Buttin,Michel Wright +1 more
Journal ArticleDOI
A Deoxyribonuclease Which Requires Nucleoside Triphosphate from Micrococcus lysodeikticus I. PURIFICATION AND CHARACTERIZATION OF THE DEOXYRIBONUCLEASE ACTIVITY
TL;DR: The results suggest that the mechanism of DNA digestion by the enzyme appears to be the one-by-one type, which is similar to that observed with pancreatic DNase.
Related Papers (5)
An ATP-dependent deoxyribonuclease from Diplococcus pneumoniae. II. Evidence for its involvement in bacterial recombination.
Gerald F. Vovis,Gérard Buttin +1 more
A Deoxyribonuclease Which Requires Nucleoside Triphosphate from Micrococcus lysodeikticus I. PURIFICATION AND CHARACTERIZATION OF THE DEOXYRIBONUCLEASE ACTIVITY
Partial purification and properties of a nucleoside triphosphate-dependent deoxyribonuclease from Mycobacterium smegmatis.
Frank G. Winder,Martin F. Lavin +1 more