The isolation and composition of two phosphoproteins from hen's egg
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Phosvitin extracted from domestic hen9s-egg yolk was resolved on Sephadex G-100 into two phosphoprotein components that differ significantly from those of the major component, and small amounts of an impurity with a much higher molecular weight are removed.Abstract:
1. Phosvitin extracted from domestic hen's-egg yolk was resolved on Sephadex G-100 into two phosphoprotein components. 2. The major component has a molecular weight of about 3.4×104 and alanine as an N-terminal residue. Glucosamine is present, but tyrosine is virtually absent. 3. The minor component has a molecular weight of about 2.8×104 and lysine as an N-terminal residue. Missing residues are glucosamine, methionine and leucine. Lysine, histidine, threonine, glycine, phenylalanine and tyrosine contents differ significantly from those of the major component. 4. Sephadex G-100 also removes small amounts of an impurity with a much higher molecular weight.read more
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Book ChapterDOI
Vitellogenesis and Oocyte Growth in Nonmammalian Vertebrates
TL;DR: The chicken egg acquires about 99% of its size during the 6 days before laying (Romanoff and Romanoff, 1949), when approximately 1 g protein is accumulated per day (Cutting and Roth, 1973).
Journal ArticleDOI
Primary activation of the vitellogenin gene in the rooster.
TL;DR: The results reported here support a model in which primary hormonal induction of vitellogenin synthesis involves activation of a dormant gene in a fully differentiated and metabolically active cell.
Journal ArticleDOI
Vitellogenin synthesis in the avian liver. Vitellogenin is the precursor of the egg yolk phosphoproteins
Roger G. Deeley,D P Mullinix,W. Wetekam,Henry M. Kronenberg,Marilyn Meyers,J D Eldridge,Robert F. Goldberger +6 more
TL;DR: It is suggested that avian vitellogenin is composed of two polypeptides, each of which has a molecular weight of approximately 240,000 and contains within it lipovitellin and two phosvitins.
Journal ArticleDOI
Estrogen-induced synthesis of yolk proteins in roosters
TL;DR: It is suggested that committed cells can synthesize vitellogenin mRNA only during a certain period of the cell cycle and the "memory" effect of estradiol is observed, which probably is due to'commitment or differentiation of viteLLogenin-synthesizing cells.