Journal ArticleDOI
Toxicity monitored with a correlated set of cell-culture assays
Ellen Borenfreund,C. Shopsis +1 more
Reads0
Chats0
TLDR
There is good agreement between these assays in the rank correlation of a broad spectrum of compounds tested, as well as with the data from Draize rabbit eye irritancy tests in vivo.Citations
More filters
Journal ArticleDOI
A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90)
TL;DR: A sensitive quantitative procedure for assaying viable cells in monolayer cultures is described, conveniently carried out within the same culture, and standardized for use with 96-well microtiter plates and automatic reading with a Dynatech spectrophotometric microplate reader.
Journal ArticleDOI
In vitro and in vivo toxicity assessment of nanoparticles
TL;DR: This review is an attempt to summarize the major techniques used in cytotoxity determination and presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles.
Journal ArticleDOI
Neutral red (NR) assay for cell viability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes.
TL;DR: Compared with lactate dehydrogenase (LDH) leakage test, the NR assay was simpler and more sensitive in determining the viability and cytotoxicity of xenobiotics in primary cultures of hepatocytes.
Journal ArticleDOI
Final report on the safety assessment of EDTA, calcium disodium EDTA, diammonium EDTA, dipotassium EDTA, disodium EDTA, TEA-EDTA, tetrasodium EDTA, tripotassium EDTA, trisodium EDTA, HEDTA, and trisodium HEDTA.
TL;DR: The Cosmetic Ingredient Review Expert Panel found that these ingredients are safe as used in cosmetic formulations and formulators should continue to be aware of this when combining these ingredients with ingredients that previously have been determined to be safe.
Journal ArticleDOI
Cytotoxicity of metals, metal-metal and metal-chelator combinations assayed in vitro
Ellen Borenfreund,J.A. Puerner +1 more
TL;DR: The neutral red assay can be a valuable tool for the screening of cytotoxic and potentially therapeutic agents under controlled in vitro conditions and showed a marked reduction of cadmium toxicity by zinc and to a lesser degree, by nickel.
References
More filters
Journal Article
Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes
Journal ArticleDOI
Evaluation of an in vitro cell toxicity test using rabbit corneal cells to predict the eye irritation potential of surfactants.
TL;DR: The in vitro method offers a means for screening potentially irritating chemicals without using live animals and may be useful in the selection of product formulations for in vivo testing.
Journal ArticleDOI
In vitro cytotoxicity assays. Potential alternatives to the Draize ocular allergy test.
Ellen Borenfreund,Olina Borrero +1 more
TL;DR: A short-term cytotoxicity assay carried out in multiwell test plates and a supplementary colony forming assay are both useful for screening and range finding of toxic concentrations of test agents.
Journal ArticleDOI
Uridine uptake inhibition as a cytotoxicity test: correlations with the Draize test.
C. Shopsis,Sonal Sathe +1 more
TL;DR: This uridine uptake assay system provides a quantitative and rapid method for assessing toxicity that correlates well with Draize test results and shares with in vivo tests the ability to demonstrate recovery from toxic insult.