Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TLDR
A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.About:
This article is published in Journal of Immunological Methods.The article was published on 1983-12-16. It has received 50114 citations till now. The article focuses on the topics: MTT assay & Chemosensitivity assay.read more
Citations
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Journal ArticleDOI
New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening
Philip Skehan,Ritsa Storeng,Dominic A. Scudiero,Anne Monks,James B. McMahon,David T. Vistica,Jonathan T. Warren,Heidi R. Bokesch,Susan Kenney,Michael R. Boyd +9 more
TL;DR: The SRB assay provides a sensitive measure of drug-induced cytotoxicity, is useful in quantitating clonogenicity, and is well suited to high-volume, automated drug screening.
Journal ArticleDOI
Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.
François Denizot,Rita Lang +1 more
TL;DR: The reliability and sensitivity of the test have been increased to the point where it can in many cases replace the [3H]thymidine uptake assay to measure cell proliferation or survival in growth factor or cytotoxicity assays.
Journal Article
Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing.
TL;DR: The clonogenic assay was more sensitive when continuous drug exposures were utilized, although this was primarily related to the increased drug exposure time, and therefore it offers a valid, simple method of assessing chemosensitivity in established cell lines.
Journal ArticleDOI
Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr-Abl positive cells.
Brian J. Druker,Shu Tamura,Elisabeth Buchdunger,Sayuri Ohno,Gerald M. Segal,Shane Fanning,Jürg Zimmermann,Nicholas B. Lydon +7 more
TL;DR: A compound, designed to inhibit the Abl protein tyrosine kinase, was evaluated for its effects on cells containing the Bcr–Abl fusion protein and it was found that this compound may be useful in the treatment of bcr–abl–positive leukemias.
Journal ArticleDOI
Porous metal–organic-framework nanoscale carriers as a potential platform for drug delivery and imaging
Patricia Horcajada,Tamim Chalati,Christian Serre,Brigitte Gillet,Catherine Sebrié,Tarek Baati,Jarrod F. Eubank,Daniela Heurtaux,Pascal Clayette,Christine Kreuz,Jong-San Chang,Young Kyu Hwang,Véronique Marsaud,Phuong-Nhi Bories,Luc Cynober,Sophie Gil,Gérard Férey,Patrick Couvreur,Ruxandra Gref +18 more
TL;DR: It is shown that specific non-toxic porous iron(III)-based metal-organic frameworks with engineered cores and surfaces, as well as imaging properties, function as superior nanocarriers for efficient controlled delivery of challenging antitumoural and retroviral drugs against cancer and AIDS.
References
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Journal ArticleDOI
Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition
TL;DR: These hybrids should prove invaluable as sources of monoclonal material for the study of the receptor(s) on T cells with H-2-restricted antigen specificities and have some bearing on models describing the nature of T cell receptors for antigen recognized in association withH-2 products.
Journal ArticleDOI
Studies on succinate-tetrazolium reductase systems. iii. points of coupling of four different tetrazolium salts.
T.F. Slater,B Sawyer,U Straeuli +2 more
TL;DR: In this article, the authors investigated the points of coupling between four tetrazolium salts and the respiratory chain (succinate to O2) in rat-liver tissue suspensions.
Journal Article
Thymoma Production of T Cell Growth Factor (Interleukin 2)
John J. Farrar,J Fuller-Farrar,Philip L. Simon,Mary L. Hilfiker,B M Stadler,William L. Farrar +5 more
TL;DR: Phorbol-12-myristate-13-acetate stimulates a subline of mouse EL-4 thymoma cells to produce, in vitro, in very high titer, T cell growth factor (Interleukin 2, IL 2), which exhibits the same spectrum of biologic activities as has been reported for spleen cell-derived IL 2.
Journal ArticleDOI
The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes
TL;DR: The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reduction in phagocytizing PMN is due exclusively to oxygen-dependent O2 - generating oxidase which is deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.
Reduction in Normal Human and Chronic Granulomatous Disease Polymorphonuclear Leukocytes
TL;DR: In this paper, a series of redox reactions takes place in polymorphonuclear teukocytes (PMN) and monocytes during phagocytosis, including consumption ofoxygen, which is insensitive to 1 mM potassium cyanide, stimulation of the hexose monophosphate shunt as determined by the evolution of ‘4C02 from gtucose-1-’4C, and the iodination of protein particles and bacteria.