Patent
Transformation of hereditary material of plants
Jerzy Paszkowski,Ingo Potrykus,Barbara Hohn,Raymond D. Shillito,Thomas Hohn,Michael W. Saul,Vaclav Mandak +6 more
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TLDR
In this paper, a novel process for the direct transfer of foreign genes to plant genomes is described, which comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.Abstract:
A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.read more
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Patent
Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof
Thomas R. Adams,Sheryl A. Chambers,Richard J. Daines,William J. Gordon-Kamm,Albert P. Kausch,Peggy G. Lemaux,Catherine J. Mackey,Mary L. Mangano,James V. O'brien,Thomas B. Rice,T. Michael Spencer,William G. Start,Nancy Willetts +12 more
TL;DR: In this article, a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed, is described. But the system is not applicable to the field of agriculture.
PatentDOI
Fertile transgenic corn plants
TL;DR: Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved as discussed by the authors.
Patent
Method for preparing fertile transgenic corn plants
TL;DR: In this article, a transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants.
Patent
Transformation and foreign gene expression in brassica species
Maurice M. Moloney,Sharon Radke +1 more
TL;DR: In this paper, the Brassica species are transformed employing a manipulated Agrobacterium transformation system, followed by regeneration of the plant tissue into plants, and the cells and the plants produced thereby are capable of expressing the foreign gene.
Patent
Control of plant gene expression
TL;DR: In this paper, a method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences.
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Journal ArticleDOI
Transformation of intact yeast cells treated with alkali cations.
TL;DR: The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
Journal ArticleDOI
Gene transfer into mouse lyoma cells by electroporation in high electric fields.
TL;DR: A simple physical model for the enhanced DNA penetration into cells in high electric fields is proposed, according to which the interaction of the external electric field with the lipid dipoles of a pore configuration induces and stabilizes the permeation sites and thus enhances cross membrane transport.
Journal ArticleDOI
Transformation of yeast
TL;DR: This work has used recently developed hybridization and restriction endonuclease mapping techniques to demonstrate directly the presence of the transforming DNA in the yeast genome and also to determine the arrangement of the sequences that were introduced.
Journal ArticleDOI
Expression of bacterial genes in plant cells.
Robert T. Fraley,Stephen G. Rogers,Robert B. Horsch,Patricia R. Sanders,Jeffery S. Flick,Steven Paul Adams,Michael L. Bittner,Leslie Brand,Cynthia L. Fink,Joyce S. Fry,Gerald R. Galluppi,Sarah B. Goldberg,Nancy L. Hoffmann,Sherry C. Woo +13 more
TL;DR: Chimeric bacterial genes conferring resistance to aminoglycoside antibiotics have been inserted into the Agrobacterium tumefaciens tumor-inducing (Ti) plasmid and introduced into plant cells by in vitro transformation techniques.
Journal ArticleDOI
Direct gene transfer to plants.
Jerzy Paszkowski,Raymond D. Shillito,Michael W. Saul,Vaclav Mandak,Thomas Hohn,Barbara Hohn,Ingo Potrykus +6 more
TL;DR: Plants regenerated from transformed cell lines were phenotypically normal and fertile, and they maintained and expressed the foreign gene throughout the development of vegetative and generative organs.