Use of a Small-Bore Internal-Surface Reversed-Phase Silica Column for the Micro High-Performance Frontal Analysis of Carbamazepine in an Albumin Solution with a Small Sample Volume.
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A micro highperformance frontal analysis (MHPFA) using a small-bore internal-surface reversed-phase silica column (10cm×1.0mm or 1.5mm id.) was developed for the simultaneous determination of the total and unbound carbamazepine (CBZ) concentrations in a human serum albumin (HSA) solution as discussed by the authors.Abstract:
A micro high-performance frontal analysis (MHPFA) using a small-bore internal-surface reversed-phase silica column (10cm×1.0mm or 1.5mm id.) was developed for the simultaneous determination of the total and unbound carbamazepine (CBZ) concentrations in a human serum albumin (HSA) solution. When a 100-μl or 200-μl portion of a 4-12μg/ml CBZ solution containing 593μM HSA was directly injected into the column, CBZ gave a trapezoidal peakwith the plateau being separated from the HSA peak. The CBZ concentration, calculated from the plateau height, agreed well with the unbound drug concentration determined by the conventional ultrafiltration-HPLC method, and the CBZ concentration calculated from the peak area coincided with the total concentration. The reproducibility was satisfactory (RSD, <3.79%, n=5 or 6). The use of a glass-lined stainless-steel column and an injector-reswitchingtechnique, which prevents the introduction of a diffused portion of the sample solution into the column, was necessary for a frontal analysis using these small-bore columns. The present MHPFA reduced the sample volume to as low as one-seventh of that required for HPFA using an internal-surface reversed-phase silica column of conventional size (15cm×4.6mm i.d.).read more
Citations
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Journal ArticleDOI
Capillary electrophoresis/frontal analysis for microanalysis of enantioselective protein binding of a basic drug
TL;DR: A new HPCE method was developed for the enantioselective determination of the unbound concentration of a basic drug under plasma protein binding equilibrium and the results obtained agreed well with those determined by conventional ultrafiltration-chiral HPLC, which was employed as a reference method.
Journal ArticleDOI
Chromatographic analysis of carbamazepine binding to human serum albumin.
Hee Seung Kim,David S. Hage +1 more
TL;DR: It was found that carbamazepine had direct competition with l-tryptophan, a probe for the indole-benzodiazepine site of HSA, but allosteric interactions with probes for the warfarin, tamoxifen and digitoxin sites.
Journal ArticleDOI
High-performance frontal analysis for drug–protein binding study
TL;DR: High-performance capillary electrophoresis/frontal analysis enables to determine unbound concentrations enantioselectively with ultramicro injection volume, and is hence useful especially for the binding study of proteins which are scarce and difficult to obtain.
Journal ArticleDOI
High-performance frontal analysis for the study of protein binding of troglitazone (CS-045) in albumin solution and in human plasma
TL;DR: An on-line frontal analysis HPLC system was developed for the determination of the unbound concentration of troglitazone (CS-045), a new oral antidiabetic agent, in human serum albumin (HSA) solution and in human plasma, and it was found that CS-045 strongly binds with HSA.
Journal ArticleDOI
Influence of glycosylation on the drug binding of human serum albumin.
Kuniko Koizumi,Chiaki Ikeda,Masae Ito,Junko Suzuki,Toshio Kinoshita,Keiko Yasukawa,Toshihiko Hanai +6 more
TL;DR: The results suggested that hydrophobic interaction is the predominant force for the drug binding in HSA, perhaps due to a conformational change or steric hindrance when further glycosylation occurred.
References
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Book
Biopharmaceutics and clinical pharmacokinetics
TL;DR: Biopharmaceutics and clinical pharmacokinetics, BiopharmACEutic drug discovery and development, and drug discovery-drug 505(B) : research and practical applications.
Book
High-Performance Liquid Chromatography: Advances and Perspectives
TL;DR: In this paper, the authors suggest to spend a few moment to read a book, even only few pages, even if it is not obligation and force for everybody, reading book becomes a choice of your different characteristics.
Journal ArticleDOI
Aqueous size exclusion chromatography
P.L. Dubin,D. Thorburn Burns +1 more
TL;DR: In this article, the authors present a separation mechanism for size exclusion in aqueous separable separable separation process (SEC) and apply it to biopolymer assemblies, and show that it can be applied to a wide range of applications.