Showing papers in "Journal of Chromatography B in 2005"

TL;DR: Evidence of oxidative stress in aging brain, some of the most important neurodegenerative diseases, and in two common and highly disabling vascular pathologies--stroke and cardiac failure are reviewed.

TL;DR: Comparisons are made of the use of 1H NMR spectroscopy and HPLC-MS for the analysis of biofluid samples and the advantages and limitations of the two approaches are assessed.

TL;DR: Improvements in LC-MS method sensitivity, speed, and resolution provided by UPLC show that further advances can be made in analytical methodology to add significant value to hypothesis-driven research.

TL;DR: In this article, a high-performance liquid chromatography (HPLC) method to determine malondialdehyde (MDA) as the 2,4-dinitrophenylhydrazine (DNPH) derivative was applied to biological samples (serum and liver homogenates).

TL;DR: An ionic liquid, 1-butyl-3-methylimidazolium chloride ([C4 mim]Cl)/salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC) for analysis of the major opium alkaloids in Pericarpium papaveris.

TL;DR: Fragmentation appears to be a kinetic process that is not caused by low levels of host cell proteases, and was not affected by host cell protein content.

TL;DR: Combination of LC-MS/MS and focused scanning for head group was revealed to be useful to identify very minor molecular species in the focused class of phospholipids.

TL;DR: Recently introduced methods for the structural analysis of released glycans (native or derivatised) as well as glycopeptides, on normal phase, reverse phase and graphitized carbon LC columns with online MS(/MS) will be reviewed.

TL;DR: This manuscript provides a review of two- and three-dimensional peptide separation strategies combined with MS for the analysis of complex peptide mixtures.

TL;DR: In this paper, the authors discuss detection-oriented derivatization as well as the application of mobile phase additives to enhance the sensitivity of detection in liquid chromatograph/atmospheric ionization/mass spectrometry (LC/API/MS), focusing in particular on the applications involving small molecules in bio-matrices.

TL;DR: Hollow fibre-based liquid-phase microextraction based on disposable hollow fibres may provide high enrichment of drugs and excellent sample clean-up, and probably has a broad application potential within the area of drug analysis.

TL;DR: The development of SPE and HPLC methods for the simultaneous determination of metformin and glipizide, gliclazide, glibenclamide or glimperide in plasma can serve as a standard method for the plasma determination therefore saving time, effort and money.

TL;DR: Liquid chromatographic/tandem mass spectrometric methods using pneumatically assisted electrospray ionisation (LC-ESI-MS/MS) was developed for determination of 18 mycotoxins and metabolites to obtain maximum recovery and minimum signal suppression/enhancement.

TL;DR: Applications of receptor-ligand binding assays described in this review will relate to screening and the quantification of pharmaceuticals in biological matrices.

TL;DR: Reversed-phase chromatography (RPLC) is the chromatographic mode most suitable for coupling with ESI-MS since mobile phases containing organic modifiers are used and Multidimensional chromatography with RPLC as a second dimension has most often been used.

TL;DR: The HPLC-MS/ESI method is accurate, sensitive and simple for routine therapeutic drug monitoring (TDM) as well as toxicologic screening, and for the study of the pharmacokinetics and metabolism of the four drugs.

TL;DR: It was found that the storage of tissue samples at -20 degrees C caused losses of dopamine and serotonin and the total time of analysis was substantially reduced resulting in a sample preparation and assay time of 15-20 min.

TL;DR: The results show that the immobilized lipase has good stability and reusability after recovery by magnetic separation within 20s, and is similar to free lipase, for which olive oil was chosen as the substrate.

TL;DR: This review summarizes all the research efforts in the last decade that have been spent to the various application of immobilized enzyme reactor (IMER) in on-line high performance liquid chromatography (HPLC) in the chemical, pharmaceutical, clinical and commodities fields.

TL;DR: The presented method can provide insights into the actual internal burden of the general population and certain risk groups and will help to further explore the human metabolism of DEHP-an occupational and environmental toxicant of great concern.

TL;DR: The HPLC fingerprint analysis is specific and may serve for quality identification and comprehensive evaluation of P. corylifolia and assess the differences among the herb grown in various areas of China.

TL;DR: The results suggest that the analysis based on fingerprint characteristics of human skin emanations could provide useful and important clues to reveal biomarkers among the mixture of humanskin emanations.

TL;DR: A specific and sensitive liquid chromatography-electrospray ionization mass spectrometry method was described for the simultaneous determination of carbamazepine, oxcarbazepine and eight of their metabolites in human plasma and found suitable for the analysis of plasma samples collected during therapeutic drug monitoring of patients treated with CBZ or OXCBZ.

TL;DR: A simple yet very effective means of minimizing the negative effect of TFA in bioanalysis by direct addition of 0.5% acetic acid or 1% propionic acid to mobile phases containing either 0.025 or 0.05% TFA is presented.

TL;DR: This review focuses on the state of the investigations of BALF proteome and its powerful contribution both to a better knowledge of the lung structure at the molecular level and to the study of lung disorders at the clinical level.

TL;DR: The procedure developed was simple, rather fast and resolutive; it permitted the recovery of these proteins with a good yield, such as 40% for cruciferin and 18% for napin, and eliminated the major phenolic compounds.

TL;DR: In this paper, the authors performed metabolic fingerprinting of rat urine by liquid chromatography-electrospray ionization mass spectrometry (LC/MS) with hydrophilic interaction liquid chromatograph.

TL;DR: The separation and purification qualities of PEI-modified magnetic nanobeads as well as its ease of use surpass those of conventional anion-exchange resins.

TL;DR: A liquid chromatographic assay with tandem mass spectrometry detection was developed for the simultaneous determination of heroin, methadone, heroin metabolites 6-monoacetylmorphine, morphine, and morphine-6 and 3-glucuronide and methad one metabolite EMDP and was successfully applied in pharmacokinetic studies with medically prescribed heroin and toxicological cases.

TL;DR: A simple, rapid, sensitive and reproducible method based on solid-phase extraction (SPE) and acidified silica clean-up was developed for the measurement of 12 polybrominated diphenyl ethers (PBDEs), including BDE 209, and 2,2',4,4',5,5'-hexabromobiphenyl (BB 153) in human serum.