Journal ArticleDOI
Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin.
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Two preservatives; zinc polyvinyl alcohol (zinc PVA) and potassium dichromate solutions for preservation of microsporidian spores separated from human faecal samples revealed that morphological features, staining characters and viability of the spores were maintained in both solutions throughout the study period.Abstract:
Microsporidia are emerging opportunistic parasites. Preservation of the biological properties of microsporidian spores is often required in research work. The present study compared two preservatives; zinc polyvinyl alcohol (zinc PVA) and potassium dichromate solutions for preservation of microsporidian spores separated from human faecal samples. After 0, 1, 2 and 4 months of storage, morphological features and staining characters of the spores were assessed by light microscopy in modified trichrome-stained smears and their viability percentages were calculated using acridine orange/ethidium bromide mixture. Also, spore infectivity was evaluated by faecal spore shedding and intestinal spore load in mice orally inoculated with the preserved spores. Results revealed that morphological features, staining characters and viability of the spores were maintained in both solutions throughout the study period. Spore infectivity was completely preserved in zinc PVA solution but showed significant reduction in potassium dichromate solution at the fourth month of the preservation duration.read more
Citations
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Journal ArticleDOI
Potentiated anti-microsporidial activity of Lactobacillus acidophilus CH1 bacteriocin using gold nanoparticles.
TL;DR: L. acidophilus CH1-derived bacteriocin can present a powerful safe therapy against intestinal microsporidiosis, as it induced a strikingly sustained reduction in fecal spore shedding after cessation of therapy by 1 week.
Journal ArticleDOI
A new scope for orlistat: Effect of approved anti-obesity drug against experimental microsporidiosis.
TL;DR: Orlistat proved its effectiveness against the intestinal microsporidial infection against both Enterocytozoon bieneusi and Encephalitozoon intestinalis by demonstrating promising antimicrosporidia potential.
References
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TL;DR: Diagnostic Parasitology Specimen Collection Specimen Processing Diagnostic Methods Organism Identification Reporting Proficiency Testing Commonly Asked Questions about Diagnostic parasitology.
Journal ArticleDOI
Zoonotic Potential of the Microsporidia
TL;DR: Vertebrate hosts are now identified for all four major microsporidial species infecting humans (E. bieneusi and the three Encephalitozoon spp.), implying a zoonotic nature of these parasites.
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Journal ArticleDOI
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Journal ArticleDOI
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