Are the mechanisms of voltage sensitivity in GEVIs closely related to protein conformation? ganapathy?
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The mechanisms of voltage sensitivity in genetically encoded voltage indicators (GEVIs) are closely related to protein conformation. Studies on the GEVI ArcLight and its variant Arclight' revealed previously unobserved features of their behavior, including millisecond-scale fluorescence fluctuations in single molecules and a delay prior to macroscopic fluorescence onset . Saturation mutagenesis experiments on a single position in the voltage-sensing domain (VSD) of a GEVI showed that amino acid substitutions at this position affected the voltage range and optical signal of the GEVI . Computational approaches have been developed to predict voltage-sensing elements in membrane proteins, independent of structure or function, by estimating their capacity to respond to changes in membrane potential . Crystal structures of the Ciona intestinalis VSD revealed reorientations of positive charges in the S4 helix, providing an explicit mechanism for voltage sensing in VSDs . These findings suggest that the conformation of the protein plays a crucial role in voltage sensitivity in GEVIs.
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220 Citations | Yes, the mechanisms of voltage sensitivity in GEVIs are closely related to protein conformation. |
| The text does not provide information specifically about GEVIs or their relationship to protein conformation. | |
8 Citations | Yes, the mechanisms of voltage sensitivity in GEVIs are closely related to protein conformation, as indicated by the effects of mutations at specific positions in the voltage-sensing domain. |
7 Citations | Yes, the mechanisms of voltage sensitivity in GEVIs are closely related to protein conformation. |
35 Citations | Yes, the mechanisms of voltage sensitivity in GEVIs are closely related to protein conformation. |
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