J
Jeffrey L. Franklin
Researcher at Vanderbilt University Medical Center
Publications - 68
Citations - 6817
Jeffrey L. Franklin is an academic researcher from Vanderbilt University Medical Center. The author has contributed to research in topics: Microvesicles & Gene. The author has an hindex of 32, co-authored 59 publications receiving 5036 citations. Previous affiliations of Jeffrey L. Franklin include Vanderbilt University & Veterans Health Administration.
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Journal ArticleDOI
Reassessment of Exosome Composition
Dennis K. Jeppesen,Aidan M. Fenix,Jeffrey L. Franklin,James N. Higginbotham,Qin Zhang,Lisa J. Zimmerman,Daniel C. Liebler,Jie Ping,Qi Liu,Rachel Evans,William H. Fissell,James G. Patton,Leonard H. Rome,Dylan T. Burnette,Robert J. Coffey,Robert J. Coffey,Robert J. Coffey +16 more
TL;DR: High-resolution density gradient fractionation and direct immunoaffinity capture are employed to precisely characterize the RNA, DNA, and protein constituents of exosomes and other non-vesicle material and show that small extracellular vesicles are not vehicles of active DNA release.
Journal ArticleDOI
The Pan-ErbB Negative Regulator Lrig1 Is an Intestinal Stem Cell Marker that Functions as a Tumor Suppressor
Anne E. Powell,Yang Wang,Yina Li,Emily J. Poulin,Anna L. Means,Mary Kay Washington,James N. Higginbotham,Alwin M. Juchheim,Nripesh Prasad,Shawn Levy,Yan Guo,Yu Shyr,Bruce J. Aronow,Kevin M. Haigis,Jeffrey L. Franklin,Robert J. Coffey,Robert J. Coffey +16 more
TL;DR: Light is shed on the relationship between proliferative and quiescent intestinal stem cells and a model in which intestinal stem cell quiescence is maintained by calibrated ErbB signaling with loss of a negative regulator predisposing to neoplasia is supported.
Journal ArticleDOI
Proteomic Analysis of Exosomes from Mutant KRAS Colon Cancer Cells Identifies Intercellular Transfer of Mutant KRAS
Michelle Demory Beckler,James N. Higginbotham,Jeffrey L. Franklin,Jeffrey L. Franklin,Amy-Joan L. Ham,Patrick J. Halvey,Imade E. Imasuen,Corbin W. Whitwell,Ming Li,Daniel C. Liebler,Robert J. Coffey,Robert J. Coffey +11 more
TL;DR: A comprehensive proteomic analysis of exosomes from parental DLD-1 cells that contain both wild-type and G13D mutant KRAS alleles and isogenically matched derivative cell lines, DKO-1 (mutant KRAS allele only) and DKs-8 (wild-type KRas allele only), showing Mutant KRAS status dramatically affects the composition of the exosome proteome.
Journal ArticleDOI
Transcriptional recapitulation and subversion of embryonic colon development by mouse colon tumor models and human colon cancer
Sergio Kaiser,Young-Kyu Park,Jeffrey L. Franklin,Richard B. Halberg,Ming Yu,Walter J. Jessen,Johannes M. Freudenberg,Xiaodi Chen,Kevin M. Haigis,Anil G. Jegga,Sue Kong,Bhuvaneswari Sakthivel,Huan Xu,Timothy Reichling,Mohammad Azhar,Gregory P. Boivin,Reade B. Roberts,Anika C. Bissahoyo,Fausto Gonzales,Greg Bloom,Steven A. Eschrich,Scott L. Carter,Jeremy Aronow,John Kleimeyer,Michael Kleimeyer,Vivek Ramaswamy,Stephen H. Settle,Braden Boone,Shawn Levy,Jonathan M. Graff,Thomas Doetschman,Joanna Groden,William F. Dove,David W. Threadgill,Timothy J. Yeatman,Robert J. Coffey,Bruce J. Aronow +36 more
TL;DR: Cross-species, developmental, and multi-model gene expression patterning comparisons provide an integrated and versatile framework for definition of transcriptional programs associated with oncogenesis and provide a general method for identifying pattern-specific biomarkers and therapeutic targets.
Journal ArticleDOI
Amphiregulin Exosomes Increase Cancer Cell Invasion
James N. Higginbotham,Michelle Demory Beckler,Jonathan Gephart,Jeffrey L. Franklin,Galina Bogatcheva,Gert-Jan Kremers,David W. Piston,Gregory D. Ayers,Russell E. McConnell,Matthew J. Tyska,Robert J. Coffey,Robert J. Coffey +11 more
TL;DR: It is speculated that EGFR ligand signaling via exosomes might contribute to diverse cancer phenomena such as field effect and priming of the metastatic niche.