How can chitosan be analyzed using NMR spectroscopy?
Best insight from top research papers
Chitosan can be analyzed using NMR spectroscopy by applying various techniques. One method involves using HOmodecoupled Band-Selective (HOBS) decoupling NMR on signals assigned by multidimensional Pure Shift NMR methods to determine the pattern of acetylation (PA) of chitosan samples . Another approach is to use chitinosanase, a sequence-specific hydrolase, in combination with ultrahigh-performance liquid chromatography–electrospray ionization-mass spectrometry (UHPLC–ESI-MS) and partial least-squares regression (PLSR) to analyze the fraction of acetylation (FA) . Additionally, spectral line shape analysis in NMR HSQC titration experiments can provide insights into the interaction between chitosan and chitosanase, allowing for the determination of equilibrium parameters and the identification of the induced-fit mechanism . Furthermore, mobility selective variable-temperature (VT) ²H solid-state NMR spectroscopy can be used to investigate the dynamics and different states of water in chitosan films, providing information about the properties and structure of the material . Solid state nuclear magnetic resonance (SSNMR) 13C-CPMAS can also be employed to analyze the structure and properties of chitosan-g-poly(N-isopropylacrylamide) (CS/PNIPAAm) and cotton-g-2-(dimethylamino) ethyl methacrylate (CG-DMAEMA) .
Answers from top 4 papers
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| Papers (4) | Insight |
|---|---|
| Chitosan can be analyzed using solid state nuclear magnetic resonance (SSNMR) spectroscopy, specifically the 13C-CPMAS technique, which provides information about the structure and properties of the biopolymer. | |
| The paper discusses the analysis of chitosan using ²H solid-state NMR spectroscopy to investigate the dynamics and organization of water in hydrated chitosan. | |
17 Citations | The paper states that chitosan can be analyzed using NMR spectroscopy by dissolving the chitosan sample in an acidic solution of D2O and recording spectra using a 300 MHz spectrometer. |
7 Citations | The paper describes a novel method using HOmodecoupled Band-Selective (HOBS) decoupling NMR and multidimensional Pure Shift NMR methods to analyze the acetylation pattern (PA) of chitosan. |
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