How can keratinocytes and C. acnes be co-cultured?
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Keratinocytes and C. acnes can be co-cultured to study interactions relevant to skin health. Studies have shown that co-culturing keratinocytes with C. acnes can lead to the activation of toll-like receptors (TLRs) 2 and 4 in keratinocytes, inducing innate immune and inflammatory responses . This co-culture model can mimic the interactions occurring in conditions like acne, where C. acnes plays a role in inflammatory acne lesions . Additionally, co-culturing keratinocytes and sebocytes, another major cell type in the pilosebaceous unit, can provide a more accurate representation of acne physiology compared to culturing either cell type alone . By utilizing co-culture models, researchers can assess inflammatory cytokine production and neutral lipid content in response to acne etiologic stimulants, aiding in understanding acne pathogenesis and screening potential treatments .
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| Papers (5) | Insight |
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17 Citations | Keratinocytes and C. acnes can be co-cultured by treating human immortalized keratinocyte cell line HPV-KER or normal human epidermal keratinocytes (NHEK) with the bacterium under specific conditions. |
Open access•Journal Article 1 Citations | Keratinocytes and C. acnes can be co-cultured by seeding immortalized human HaCaT keratinocytes and SZ95 sebocytes in 24-well culture plates and treating them with acne etiologic stimulants. |
| Not addressed in the paper. | |
| Keratinocytes and C. acnes can be co-cultured on a 3D reconstructed human epidermis model, allowing for the study of interactions mimicking those in dandruff conditions. | |
| Not addressed in the paper. |
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