scispace - formally typeset
Search or ask a question

How does extraction oil bodies using enzyme? 


Best insight from top research papers

Extraction of oil bodies using enzymes involves breaking down the cell wall structure to release oil bodies efficiently. Enzymes like cellulase, pectinase, and Viscozyme® L play crucial roles in this process by degrading components of the cell wall . The use of specific enzymes affects the yield and stability of oil bodies, with optimal conditions enhancing extraction rates. Enzymatic extraction methods also impact the composition and properties of oil bodies, such as protein content, fatty acid composition, and stability . Techniques like confocal laser scanning microscopy and liquid chromatography-tandem mass spectrometry aid in observing the structure and identifying proteins within oil bodies . Overall, enzymatic extraction of oil bodies is a promising approach for efficient oil recovery with potential applications in various industries.

Answers from top 5 papers

More filters
Papers (5)Insight
Proteases hydrolyze cotyledon cell walls in enzyme-assisted aqueous extraction, aiding in releasing oil from oil bodies, resulting in high oil recovery and economic feasibility in soybean oil production.
Oil bodies are extracted using enzymes like Viscozyme® L in aqueous enzymatic extraction. Optimal conditions include enzyme hydrolysis at 52°C, solid-liquid ratio of 1 : 4, enzyme concentration of 1.35%, and hydrolysis time of 90 min.
Aqueous enzymatic extraction with cellulase and pectinase disrupts cell walls, yielding 90.7% oil bodies. This method enhances oil body stability and extraction efficiency for commercial applications.
Enzyme extraction of rice bran oil bodies involves using plant-extracted enzyme, xylanase, or their mixture, resulting in uniform distribution due to high protein content and steric hindrance.
Enzymatic hydrolysis of the peanut cell wall using Viscozyme® L degrades cellulose, hemicellulose, and pectin, facilitating the extraction of oil bodies and peanut protein.

Related Questions

How enzyme is used in determination of water contamination?5 answersEnzymes play a crucial role in the determination of water contamination through various applications. Enzyme-based nanosensors offer a sensitive and stable method for detecting pollutants in water, providing a reliable tool for environmental monitoring. Enzymatic actions in biological treatment methods offer advantages over chemical and physical strategies, promoting clean and biodegradable approaches for wastewater treatment. Enzyme-based biosensors are highlighted as effective tools for real-time monitoring of toxic elements in water, with successful applications in detecting a wide range of pollutants at low concentrations. Novel parameters like enzymatic activity of beta-D-glucuronidase are introduced for rapid measurement of fecal bacteria contamination in water, enabling efficient water quality monitoring and process control applications. Automated on-line monitoring of enzymatic activities, such as beta-D-glucuronidase, showcases the potential for microbial water quality monitoring, although further research is needed to establish its full capacity as a rapid indicator of microbial contamination in water sources.
What are the disadvantages of using ethanol prebiotic extraction with enzyme-assisted extraction?5 answersThe disadvantages of using ethanol prebiotic extraction with enzyme-assisted extraction include low selectivity, poor yield, toxicity, and potential diminishing of bioactivity after extraction. Additionally, traditional methods like solvent extraction or maceration suffer from drawbacks such as low extraction rates, long extraction times, and high energy consumption. Enzyme-assisted extraction methods, on the other hand, offer advantages like increased extraction yield, improved production efficiency, reduced energy costs, and visually appealing final products in fruit juice manufacturing. Enzymes play a crucial role in breaking down complex polysaccharides in fruit tissues, enhancing the extraction process and overall product quality. Furthermore, enzyme-assisted extraction can be applied to various industrial domains, including pharmaceuticals, food, and specialty chemicals, due to its eco-friendly and sustainable nature.
What enzyme is used in the enzymatic extraction of P(3HB)?5 answersThe enzyme used in the enzymatic extraction of P(3HB) is Celumax® BC.
How to extract oil from fish?5 answersFish oil can be extracted using various methods such as supercritical fluid extraction (SFE-CO2), enzymatic hydrolysis extraction after ultra-high pressure pre-treatment (EHSUP), modified soxhlet extraction, and ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE). In SFE-CO2, the oil is extracted using supercritical conditions of time, temperature, and pressure. EHSUP involves pre-treating the fish heads with ultra-high pressure before enzymatic hydrolysis. Modified soxhlet extraction uses solvents like ethanol to extract the oil. UAE and MAE are novel technologies that utilize ultrasound and microwave to extract oil from fish by-products. Each method has its own advantages in terms of oil yield, fatty acid composition, and extraction efficiency.
How to combine deep eutectic solvent and enzyme to extraction of phenolic compounds?5 answersDeep eutectic solvents (DESs) have been used in combination with ultrasound-assisted extraction for the recovery of phenolic compounds from various sources such as apple pomace, olive leaves, and brewer's spent grain (BSG). Choline chloride-based DESs have been particularly effective in the extraction of phenolic compounds. The use of DESs has shown higher extraction yields compared to conventional solvents like ethanol. The extraction process using DESs can be optimized by adjusting parameters such as temperature, water addition, and extraction time. Additionally, DESs have been found to be environmentally friendly and non-toxic alternatives to conventional solvents. The combination of DESs with microwave assistance has also been explored for the extraction of phenolic compounds. However, the specific combination of deep eutectic solvents and enzymes for the extraction of phenolic compounds was not mentioned in the provided abstracts.
What are the experimental methods other than enzyme assisted extraction are carried out for valorization of red capsicum?3 answersDifferent experimental methods other than enzyme-assisted extraction have been carried out for the valorization of red capsicum. These methods include distillation techniques such as steam distillation and simultaneous distillation extraction. Another method is Soxhlet extraction, where capsicum powder is extracted using tetrahydrofuran at specific temperatures and times, followed by reduced pressure filtering and distillation. Additionally, aqueous carotenoid-rich extract (ACE) has been developed through enzymatic liquefaction, which significantly improves the extract yield and total carotenoid content. Capsicum oleoresins have also been extracted using various methods such as water extraction, organic solvent extraction, microwave-assisted extraction, and ultrasound-assisted extraction. These methods are often preceded by pretreatments like chemical treatments, osmotic dehydration, and drying techniques. Overall, these different experimental methods contribute to the valorization of red capsicum by extracting valuable compounds such as carotenoids and pigments.

See what other people are reading

What are industrial applications of hemp fibre?
4 answers
Industrial applications of hemp fiber are diverse and expanding globally. Hemp fiber, rich in cellulose derivatives, is utilized in various industries such as food, textile, and packaging. It is a key component in the production of composite materials, where physical and chemical modifications enhance mechanical and barrier properties. Additionally, hemp fiber is increasingly used in the construction sector, particularly in lightweight foamed concrete, to improve mechanical properties like compressive strength and flexural strength. The sustainability of hemp as a plant is highlighted, as it requires fewer pesticides and water compared to other fiber plants like cotton, making it an environmentally friendly choice for industrial applications. The versatility of hemp fiber extends to the creation of high-value goods in the food, nutraceutical, and cosmeceutical sectors, promoting a circular supply chain and novel applications.
What are examples of industrial applications of hemp fibre?
5 answers
Industrial applications of hemp fiber include its use in food, textile, packaging, construction materials, and essential oil production. Hemp fibers, rich in cellulose derivatives, are utilized for reinforcement in thermoplastic packaging and paper. Research shows that combining natural and synthetic fibers, like hemp, in lightweight foamed concrete enhances mechanical properties, such as compressive strength and flexural strength. Additionally, hemp derivatives from legal Cannabis sativa L. are used in various industrial applications, with the essential oil extracted from hemp showing promise in fields like pest management, pharmaceuticals, and cosmetics. The development of green composites using natural fibers, particularly hemp, aids in reducing the carbon footprint of composite materials, contributing to a more sustainable environment. Overall, hemp's versatility and sustainability make it a valuable resource for a wide range of industrial applications.
What is new in fermetation of cascara (husk coffee)?
5 answers
The latest advancements in the fermentation of cascara (coffee husk) involve utilizing it as a substrate for kombucha production, resulting in a novel beverage with health benefits. Research has shown that cascara kombucha fermentation enhances antioxidant, antimicrobial, and prebiotic activities, promoting a healthy gut microbiota. Additionally, studies have explored the optimal coupling mode of chemical fertilizer application and coffee husk return methods to improve soil fertility and promote photosynthetic efficiency of Arabica coffee trees, emphasizing the benefits of coffee husk biochar and compost. Furthermore, investigations have focused on the quality of kombucha cascara by fermenting different varieties of arabica coffee for varying durations, highlighting significant effects on antioxidant activity, pH, and microbial content. These recent findings underscore the potential of cascara fermentation in creating innovative and beneficial products.
Can biodegradable films be used effectively in various industries such as food packaging, agriculture, and medical applications?
5 answers
Biodegradable films have shown great potential for effective utilization across various industries. In the food packaging sector, biodegradable films offer eco-friendly alternatives with enhanced properties through additives like nanomaterials and bioactive compounds. Additionally, in agriculture, these films can contribute to sustainability by providing protective and functional packaging for agricultural products, thus extending their shelf life. Moreover, in medical applications, biodegradable films based on biopolymers have been explored for their biocompatibility and biodegradability, making them suitable for controlled drug delivery systems and wound dressings. The versatility and environmentally friendly nature of biodegradable films make them promising candidates for diverse industrial applications, including food packaging, agriculture, and medical sectors.
What is the degree celsius to convert walnut shell to walnut ash?
5 answers
To convert walnut shell to walnut ash, the pyrolysis temperature plays a crucial role. Research indicates that walnut shells are best pyrolyzed at different temperatures depending on the desired application. Walnut shells are suitable for use as alternative fuels when pyrolyzed at 300 °C for soil fertilizers, while pistachio shells are optimal at 350 °C and peanut shells at 550 °C. Additionally, the pyrolysis temperature for bio-char production from walnut shells is recommended to be 600 °C for improved High Heating Value and carbon content. Furthermore, the optimal carbonization process for walnut shells involves a final temperature of 324.7 °C, with the carbonization reaction intensifying at a heating rate of about 10 °C min−1.
Is kluyveromyces marxianus top or bottom fermenting yeast?
5 answers
Kluyveromyces marxianus is a top-fermenting yeast, unlike Saccharomyces cerevisiae, which is bottom-fermenting. K. marxianus strains are generally Crabtree-negative or exhibit aerobic-respiring characteristics, indicating their inability to undergo aerobic alcoholic fermentation, a trait associated with bottom-fermenting yeasts like S. cerevisiae. Additionally, K. marxianus CBS 6556 has been highlighted as a promising host for simultaneous saccharification and fermentation (SSF) processes due to its thermotolerance, thriving at 43 °C, making it suitable for high solids fermentation, a characteristic not typical of bottom-fermenting yeasts like S. cerevisiae. Therefore, based on the characteristics and behaviors described in the research papers, Kluyveromyces marxianus can be classified as a top-fermenting yeast.
Is banana leaves viable as an ingredient in organic glue.?
5 answers
Banana leaves have shown potential for various applications, including organic glue production. Studies have highlighted the utilization of banana leaf wax as a natural material for industrial purposes, indicating its feasibility as a component in organic glue due to its chemical composition and properties. Additionally, the extraction of bioactive constituents from banana peels has been explored for developing bio-composite organic coatings with corrosion inhibition properties, showcasing the effectiveness of banana-derived additives in enhancing material properties. Furthermore, the investigation of banana fibres treated with different agents demonstrated changes in morphology and chemical composition, suggesting the possibility of utilizing banana fibres in adhesive applications. Overall, the data supports the viability of banana leaves and their derivatives as ingredients in organic glue formulations.
What useful products can be made from biomass waste and applied in agriculture?
5 answers
Biomass waste holds significant potential for producing valuable products applicable in agriculture. Various sources like wood waste, agricultural biomass waste (ABW), and cellulose from different sources can be utilized. Wood waste can be transformed into organic amendments, poultry bedding, renewable energy sources, and construction materials. ABW, when processed through microwave-assisted pyrolysis (MAP), can yield biochar beneficial for soil fertilization, enhancing soil fertility, water retention, and crop productivity. Additionally, cellulose from waste biomass can be converted into nanocellulose with unique properties, suitable for applications in nanocomposites, biomedical fields, and packaging, offering a sustainable solution to environmental challenges. These products not only add value to biomass waste but also contribute to sustainable agricultural practices and environmental conservation.
Selection of stationary and mobile phase of HPLC?
10 answers
The selection of stationary and mobile phases in high-performance liquid chromatography (HPLC) is crucial for achieving optimal separation of analytes based on their chemical and physical properties. Stationary phases with specific characteristics are chosen to interact with the analytes in desired ways. For instance, the use of amylose-based chiral stationary phases (CSPs) has proven effective for the enantioseparation of compounds, demonstrating the importance of selecting stationary phases that match the stereogenic elements of the analytes. Similarly, novel stationary phases such as those with embedded hydrophilic groups or nickel oxide deposited silica have been developed to cater to specific separation needs, including the use of pure water as a mobile phase and enhancing chromatographic selectivity through multiple retention mechanisms. The choice of mobile phase is equally significant and is influenced by the nature of the stationary phase and the analytes. The trend towards green chemistry has led to the exploration of environmentally benign mobile phases, such as pure water, supercritical carbon dioxide, and ethanol, to reduce the production of harmful waste. The use of pure water is particularly noteworthy for its role in reducing organic solvent waste and its compatibility with new stationary phases designed for aqueous separations. Moreover, the mobile phase composition can be tailored to enhance the separation of chiral compounds by incorporating chiral mobile phase additives (CMPAs), which form transient diastereomeric complexes with analytes, facilitating enantioseparation on achiral stationary phases. The versatility of HPLC is further demonstrated by the use of biodegradable polymers like chitosan for stationary phases, offering a sustainable and cost-effective alternative with comparable performance to conventional columns. In summary, the selection of stationary and mobile phases in HPLC is a multifaceted decision process that considers the chemical nature of the analytes, the desired separation mechanism, and environmental impact. Advances in stationary phase design and mobile phase composition continue to expand the capabilities and applications of HPLC in analytical chemistry.
Other matrices used for bioplastic development?
4 answers
Various matrices have been explored for bioplastic development. Cellulose and starch are highlighted as potential bioplastic raw materials due to their environmental benefits. Additionally, bio-based matrices like vegetable oils, cashew nut shell liquid, polysaccharides, polylactide (PLA), polyhydroxyalkanoates (PHAs), proteins, and lignin have shown promise in biocomposite applications, offering good mechanical properties and environmental advantages. Soy protein isolate (SPI) and egg white albumen concentrate (EW) have been utilized to create bioplastics with differing properties, where SPI bioplastics exhibit enhanced water uptake behavior and ductility, making them suitable for superabsorbent applications, while EW bioplastics offer higher mechanical properties. These diverse matrices contribute to the advancement of bioplastics with varied characteristics and applications.
Why are there studies combining starch and pectin together for bioplastic making (starch/pectin matrix)?
5 answers
Studies combine starch and pectin for bioplastic making to enhance properties like mechanical strength, water absorption, and encapsulation efficiency. Pectin, a common wall material, when combined with starch, improves matrix retention and controlled release. Starch, known for its potential in bioplastics, can be modified to enhance mechanical properties. Additionally, the use of Deep Eutectic Solvent (DES) and citric acid as plasticizers and crosslinkers respectively, enhances the properties of pectin-based bioplastics, making them more flexible and environmentally friendly. Furthermore, blending starch from different sources and crosslinking with citric acid alters the physicochemical and functional properties of the bioplastics, making them more sustainable and efficient. Combining starch and pectin offers a synergistic approach to overcome the limitations of individual biopolymers, leading to the development of improved bioplastic materials.