How to differentiate pseudomonas aeruginosa with other bacteria as grown on cetrimide agar?
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To differentiate Pseudomonas aeruginosa from other bacteria grown on cetrimide agar, various methods can be employed. One approach involves utilizing selective media containing specific additives like benzalkonium chloride (BKC) to inhibit the growth of accompanying flora. Additionally, molecular techniques such as PCR assays targeting genus and species-specific 16S rRNA signature sequences can be utilized for accurate identification of P. aeruginosa. Another innovative method involves detecting volatile organic compounds (VOCs) using multi-capillary column-ion mobility spectrometry, which has shown promise in differentiating between P. aeruginosa and other bacteria. Moreover, the development of new selective agar media like CKNA, containing cetrimide, kanamycin sulfate, and nalidixic acid, has demonstrated high sensitivity and specificity rates for isolating P. aeruginosa from clinical specimens.
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| Papers (5) | Insight |
|---|---|
| Pseudomonas aeruginosa can be differentiated from other bacteria on cetrimide agar by its pigment production, with CKNA agar showing the highest sensitivity and specificity among tested media. | |
Open access•Journal Article 14 Citations | Using 16S rRNA gene sequencing allows for accurate differentiation of Pseudomonas aeruginosa from other bacteria grown on cetrimide agar, providing a more specific identification method compared to traditional tests. |
07 Feb 2022 | Using multi-capillary column-ion mobility spectrometry (MCC-IMS) to detect volatile organic compounds (VOCs) in the headspace over bacteria grown on cetrimide agar allows differentiation of Pseudomonas aeruginosa from other bacteria. |
2 Citations | Pseudomonas aeruginosa can be differentiated from other bacteria on Cetrimide agar by adding 375 μg/mL BKC and incubating at 42°C to suppress accompanying flora. |
| Pseudomonas aeruginosa on cetrimide agar can be differentiated by its characteristic fluorescence under UV light, aiding in distinguishing it from other bacteria present in the culture. |
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