How to increase hplc resolution?
Best insight from top research papers
To increase HPLC resolution, there are several methods that can be employed. One approach is to operate small-particle columns at elevated temperatures, which decreases mobile phase viscosity and enhances solute diffusion, resulting in increased column plate number and separation resolution . Another method is to use multidimensional HPLC, which involves in-depth optimization of selectivity and is effective for handling complex mixtures . However, if the samples have extreme complexity and only need to be separated once, a one-dimensional approach can be used. This approach involves using very long columns packed with conventional 5 μm superficial porous materials or shorter columns with circular flow (recycling chromatography) to achieve peak capacity beyond UHPLC . Additionally, coupling multiple columns packed with 5 μm spherical ROSiL-C18-D silica gel can also improve HPLC resolution .
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| Papers (5) | Insight |
|---|---|
4 Citations | The paper suggests that to increase HPLC resolution, one can use multidimensional HPLC and optimize selectivity. However, for samples of extreme complexity that only need to be separated once, a one-dimensional approach with very long columns or recycling chromatography can be used. |
11 Citations | The paper discusses three approaches to increase HPLC resolution: coupling of multiple packed columns, using microbore packed columns, and using capillary (open tubular) columns. |
7 Citations | The provided paper is about increasing the spatial resolution in digital holographic microscopy, not about increasing HPLC resolution. Therefore, the paper does not provide information on how to increase HPLC resolution. |
Open access•Journal Article 27 Citations | The paper suggests that operating small-particle columns at elevated temperatures can increase the resolution in HPLC separation of peptides. |
| The provided paper is about increasing mass spectrum resolution, not HPLC resolution. The paper does not provide information on how to increase HPLC resolution. |
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