What are the key enzymes in cephalosporin biosynthesis?
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Key enzymes in cephalosporin biosynthesis include Deacetoxycephalosporin C synthase (DAOCS), Deacetylcephalosporin C synthase (DACS), and Cephalosporin-C acylase (CPC acylase) . DAOCS catalyzes the oxidative expansion of the penicillin nucleus to the cephalosporin nucleus, while DACS hydroxylates the cephalosporin product at the 3'-position. DACS is crucial for transforming cephalosporin intermediates into valuable compounds like 7-amino-cephalosporanic acid (7ACA) and hydroxymethyl-7-amino-cephalosporanic acid (HACA) . Additionally, CPC acylase plays a vital role in converting cephalosporin-C to 7-ACA, a key intermediate in semisynthetic cephalosporin production . These enzymes are essential for the efficient biosynthesis of cephalosporins, highlighting their significance in the pharmaceutical industry.
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| Key enzymes in cephalosporin biosynthesis include DAC acetyltransferase (encoded by cefG) and isopenicillin N acyltransferase, crucial for the conversion of intermediates to cephalosporin. | |
23 Apr 2015 1 Citations | Key enzymes in cephalosporin biosynthesis include DAOCS for ring expansion, DACS for hydroxylation, and CmcI/J for methoxylation, all utilizing 2OG-dependent oxygenases for catalysis. |
| Cephalosporin-C acylase (CPC acylase) is a key enzyme in cephalosporin biosynthesis, converting cephalosporin-C to 7-aminocephalosporanic acid (7-ACA), a crucial intermediate for semisynthetic cephalosporin antibiotics. | |
2 Citations | Deacetylcephalosporin C synthase (DACS) is a crucial enzyme in cephalosporin biosynthesis, transforming deacetoxycephalosporin C (DAOC) into deacetylcephalosporin C (DAC) during the process. |
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