What is the optimal temperature range for fermenting acetic acid with acetobacter aceti?4 answersThe optimal temperature range for fermenting acetic acid with Acetobacter aceti varies depending on the specific strain and conditions. Acetification by Acetobacter aceti WK for gluconic acid production showed successful results at 37°C, yielding high concentrations of acetic acid and gluconic acid. Additionally, thermally adapted strains of Acetobacter pasteurianus were able to grow well in nutrient-poor media at temperatures up to 40˚C, showcasing high-temperature fermentation abilities. Furthermore, Acetobacter pasteurianus strain SRCM101388 demonstrated optimal conditions for vinegar fermentation at 28°C. These findings suggest that the optimal temperature range for fermenting acetic acid with Acetobacter aceti and related species can vary from around 28°C to 40°C, depending on the specific strain and fermentation goals.
What are the optimal culture conditions of Faecalibacterium?5 answersThe optimal culture conditions for Faecalibacterium involve factors like temperature, pH, and incubation time. Studies have shown that Faecalibacterium prausnitzii growth can be inhibited by calcium palmitate (CP). Additionally, Enterococcus feacium CST-1 displayed increased antibacterial activity after optimization of medium components like carbon and nitrogen sources, temperature, and pH. Furthermore, research on high-density culture conditions of Lactobacillus demonstrated that environmental conditions and medium composition significantly impacted bacterial growth, with biomass reaching up to 35.4 g/L under optimized conditions. Optimum conditions for growth and bacteriocin production in Ent. faecium were found to be pH 8 and 37°C, respectively. These findings collectively emphasize the importance of optimizing culture conditions to enhance the productivity and effectiveness of Faecalibacterium and related bacterial strains.
How to optimize the nitrogen source in probiotic bacteria?4 answersTo optimize the nitrogen source in probiotic bacteria, several studies have been conducted. Barbeau et al. found that substituting the nitrogen content of the culture media with alternative nitrogen sources, such as X-Seed KAT, X-Seed Carbo Max, and X-Seed Nucleo Max, resulted in significantly higher bacterial growth in Lactobacillus bulgaricus. Similarly, Chen et al. used the Central Composite Design (CCD) to optimize the nitrogen source for Bifidobacterium bifidum BB01 and found that a composition of peptone, yeast extracts, and beef paste resulted in higher viable counts. These studies suggest that the nitrogen source composition plays a crucial role in enhancing the growth of probiotic bacteria. By modifying the nitrogen content of the culture media and using alternative nitrogen sources, it is possible to optimize the growth conditions for probiotic bacteria and improve their viability and biomass production.
Will Azotobacter die at high temperature?5 answersAzotobacter is sensitive to high temperatures above 35 °C. Inactivation of the arfB gene in Azotobacter vinelandii resulted in the strain being unable to grow after 2 hours of incubation at 45°C. The growth of Azotobacter vinelandii NDD-CK-1 decreased with increasing temperature. These findings suggest that Azotobacter can die at high temperatures, particularly above 45°C.
What are the optimum temperatures for growth of Salmonella Typhimurium?5 answersThe optimum temperatures for growth of Salmonella Typhimurium vary depending on the context. In one study, the proteome profile of Salmonella Typhimurium grown in rich medium (LB medium) or minimal medium (PCN medium) at temperatures ranging from 8 °C to 37 °C was analyzed. It was found that Salmonella Typhimurium can grow at temperatures as low as 8 °C, which is the temperature typically found during food storage. Another study found that the growth dynamics of Salmonella Typhimurium as a function of temperature were studied in petri dishes. It was observed that the growth rates for Salmonella Typhimurium were almost equal for both planktonic cells and colonies, regardless of the temperature. Additionally, a study investigating the effect of pH and temperature on the survival of Salmonella Typhimurium in mayonnaise found that the survival of the bacteria was significantly improved at 4 °C. Therefore, the optimum temperatures for growth of Salmonella Typhimurium can range from 8 °C to 37 °C, depending on the specific conditions.
What is the optimal temperature for fermentation using Hanseniaspora uvarum?5 answersThe optimal temperature for fermentation using Hanseniaspora uvarum is 28°C.