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What are the optimal growth temperature for Cupriavidus necator?

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The optimal growth temperature for Cupriavidus necator varies depending on the specific conditions and objectives. Research by Ren et al. found that for polyhydroxyalkanoate (PHA) production, the optimal temperature was 25°C . On the other hand, Boy et al. demonstrated that a temperature increase from 30°C to 37°C was the most efficient strategy for generating plasmid expression level heterogeneity . Additionally, Lambauer et al. highlighted the importance of precise oxygen levels for autotrophic growth, with dissolved O2 concentrations adjusted to 1%, 2%, and 4% saturation for optimal cell dry weight and poly-(R)-3-hydroxybutyrate content . Therefore, the optimal growth temperature for Cupriavidus necator can range from 25°C for PHA production to 37°C for inducing plasmid expression level heterogeneity and precise oxygen levels for autotrophic growth.

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Journal Article•DOI Optimization of the chemolithoautotrophic biofilm growth of Cupriavidus necator by means of electrochemical hydrogen synthesis S. Bause, M. Decker, Peter Neubauer, Winfried Vonau - Show less +3 more 15 Jan 2018-Chemical Papers 4 Citations	Not addressed in the paper.
Open access•Journal Article•DOI Automatic Control of Chemolithotrophic Cultivation of Cupriavidus necator: Optimization of Oxygen Supply for Enhanced Bioplastic Production Vera Lambauer, Alexander Permann, Zdeněk Petrášek, Vanja Subotić, Christoph Hochenauer, Regina Kratzer, Markus Reichhartinger - Show less +6 more 29 Jun 2023-Fermentation	The paper does not address the optimal growth temperature for Cupriavidus necator.
Journal Article•DOI Synthesis of Polyhydroxyalkanoates from Waste Frying Oil by Cupriavidus necator Lian Hai Ren, Hui Liu, Pan Wang - Show less +2 more 01 Jun 2015-Applied Mechanics and Materials	The optimal growth temperature for Cupriavidus necator is 25°C.
Journal Article•DOI Study of plasmid-based expression level heterogeneity under plasmid-curing like conditions in Cupriavidus necator. Catherine Boy, Julie Lesage, Sandrine Alfenore, Stéphane E. Guillouet, Nathalie Gorret - Show less +4 more 01 Jan 2022-Journal of biotechnology 1 Citations	The optimal growth temperature for Cupriavidus necator is not mentioned in the provided paper.

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How can polymer be produced from cupriavidus necator?5 answersPolymer can be produced from Cupriavidus necator through the synthesis of polyhydroxyalkanoates (PHAs) using different carbon substrates and precursors. The production of P(3HB-co-3HHx) copolymers with varying compositions of 3HHx monomers can be enhanced by optimizing factors such as crude palm kernel oil (CPKO) concentration, sodium hexanoate concentration, and cultivation time. Another study found that ε-caprolactone is the best precursor for the synthesis of P(3HB-co-4HB) copolymers, and by varying the concentration and number of doses of ε-caprolactone, copolymers with different contents of 4HB can be obtained. Additionally, a synthetic entry into the metabolic pathway of bio-polyester synthesis of Cupriavidus necator H16 has been created, enabling the production of aliphatic and aromatic bio-polyesters with properties similar to synthetic polyesters like PET and PEF. Furthermore, Cupriavidus necator can utilize CO2 as a carbon source and H2 as an electron donor to produce poly-(R)-3-hydroxybutyrate, with the optimal dissolved O2 concentration being between 0.2 and 4 mg/L.

What is the optimum temperature for growth of S. aureus?4 answersThe optimum temperature for the growth of S. aureus is 40.6°C.

Has endotoxin levels in Cupriavidus necator been measured?4 answersEndotoxin levels in Cupriavidus necator have been measured in the study conducted by Lange de Pinho Neves et al.. The study aimed to compare the viability of preserving Cupriavidus necator using sterilized long-life skim milk (LDEA) and commercially sterilized long-life skim milk (LDCE) as protectants. The viability of the bacterial cells protected with LDEA was found to be 7.0% survival, while for LDCE, it was 12.7% survival. The study concluded that skim milk had a protective effect on Cupriavidus necator, with LDCE showing a higher protection capacity during lyophilization for the studied microorganism. However, the survival rates were relatively low compared to other microorganisms in the literature. No other abstracts mention the measurement of endotoxin levels in Cupriavidus necator.

What's the optimum ph, temperature, salinity, and oxygen levels for l. acidophilus?4 answersThe optimum pH for L. acidophilus is 7.5. The optimum temperature for L. acidophilus is 70 °C. The optimum salinity for L. acidophilus is not mentioned in the provided abstracts. The optimum oxygen levels for L. acidophilus are not mentioned in the provided abstracts.

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