What are the potential applications of Botrytis cinerea research in the development of novel biotechnological tools and processes?
Best insight from top research papers
Research on Botrytis cinerea, a significant phytopathogenic fungus, has unveiled various potential applications in the development of novel biotechnological tools and processes. The development of the BEB, a web-based B. cinerea gene expression browser, facilitates the analysis and visualization of gene expression patterns in B. cinerea, offering a platform for identifying genes involved in pathogenicity and resistance, which could be targeted in crop protection strategies . Additionally, the study of succinate dehydrogenase inhibitors (SDHIs) resistance in B. cinerea provides insights into managing fungicide resistance, crucial for developing new fungicidal compounds or strategies to mitigate resistance . The exploration of B. cinerea proteases for the enzymatic degradation of heat haze-forming proteins in winemaking presents an environmentally friendly alternative to traditional methods, reducing labor costs and environmental impacts . Furthermore, the investigation into natural compounds like perillaldehyde demonstrates its potential as a safe and effective antifungal agent, offering a sustainable approach to managing gray mold disease . Research into B. cinerea's ability to tolerate and detoxify phytoalexins through efflux and enzymatic detoxification mechanisms opens avenues for understanding fungal resistance mechanisms and developing novel fungicides . The study of B. cinerea's metabolism of sesquiterpenoid phytoalexins and the identification of specific detoxification genes provides insights into the pathogen's host range and virulence, which could inform crop breeding programs for resistance . The characterization of the B. cinerea surfactome, identifying potential pathogenicity and virulence factors, offers targets for fungicide development, contributing to the control of this pathogen . Lastly, the analysis of small RNAs in B. cinerea through high-throughput sequencing provides a foundation for understanding the fungus's developmental and pathogenic mechanisms at the transcriptional level, which could be exploited in biotechnological applications . Collectively, these studies highlight the multifaceted potential of B. cinerea research in advancing biotechnological tools and processes for agricultural and environmental benefits.
Answers from top 10 papers
More filters
| Papers (10) | Insight |
|---|---|
11 May 2022 | Research on Botrytis cinerea's surfactome can aid in identifying new pathogenicity factors, enhancing fungicide development, and advancing biotechnological tools for crop protection and disease management. |
| Research on Botrytis cinerea's surfactome can aid in identifying new pathogenicity factors, enhancing fungicide development, and advancing biotechnological tools for crop protection and disease management. | |
| Research on Botrytis cinerea's surfactome can aid in identifying new pathogenicity factors, enhancing fungicide development, and advancing biotechnological tools for crop protection and disease management. | |
| Research on Botrytis cinerea's surfactome can aid in identifying new pathogenicity factors, enhancing fungicide development, and advancing biotechnological tools for crop protection and disease management. | |
| Research on Botrytis cinerea's surfactome can aid in identifying new pathogenicity factors, enhancing fungicide development, and advancing biotechnological tools for crop protection and disease management. | |
| Research on Botrytis cinerea's surfactome can aid in identifying new pathogenicity factors, enhancing fungicide development, and advancing biotechnological tools for crop protection and disease management. | |
| Research on Botrytis cinerea's surfactome can aid in identifying new pathogenicity factors, enhancing fungicide development, and advancing biotechnological tools for crop protection and disease management. | |
| Botrytis cinerea research can aid in developing novel biotechnological tools by analyzing gene expression patterns, secondary metabolite clusters, virulence factors, and reference genes for potential applications in biotechnology. | |
| Botrytis cinerea research can lead to novel biotechnological tools by analyzing gene expression patterns, aiding in understanding secondary metabolite clusters, virulence factors, and reference genes for biotechnological advancements. | |
1 Citations | Research on Botrytis cinerea can lead to the development of molecular diagnostic tools for detecting SDHI resistance mutations, aiding in effective gray mold disease control in agriculture. |
Related Questions
How does the use of juniper essential oil compare to synthetic chemical pesticides in controlling Botrytis cinerea?5 answersJuniper essential oil (JEO) demonstrates significant efficacy in controlling Botrytis cinerea, a plant pathogenic fungus, comparable to synthetic fungicides. JEO inhibits spore germination and mycelial growth of B. cinerea in a concentration-dependent manner, reducing disease incidence and lesion diameters on cherry tomato fruit. Additionally, JEO damages fungal membranes, alters mycelial morphology, and modulates enzyme activities, enhancing antioxidant defenses while reducing pathogenicity-related enzymes. Similarly, other essential oils like savory and peppermint exhibit potent antifungal activity against B. cinerea, affecting cell membrane permeability and electrolyte leakage. Furthermore, botanical extracts, including neem and ginger, along with biocontrol agents like Fusarium solani, show promising results in inhibiting B. cinerea growth, suggesting their potential as eco-friendly alternatives to synthetic fungicides. These findings collectively highlight the effectiveness of natural compounds like JEO and essential oils in combating B. cinerea, offering safer and sustainable pest management solutions.
How does the application of juniper essential oil affect the growth and survival of Botrytis cinerea in forest seedling?5 answersThe application of juniper essential oil (JEO) has been shown to significantly inhibit the growth and survival of Botrytis cinerea, a plant pathogenic fungus. Studies have demonstrated that JEO inhibits spore germination and mycelial growth of B. cinerea in a concentration-dependent manner, with concentrations exceeding 10 μL/mL exhibiting strong inhibition. Additionally, JEO damages fungal membranes, alters mycelial morphology, and reduces the activity of pathogenicity-related enzymes in B. cinerea. Furthermore, JEO contains various antimicrobial compounds like limonene, terpinen-4-ol, and linalool, contributing to its antifungal properties. These findings suggest that the application of juniper essential oil can be an effective natural alternative for controlling Botrytis cinerea in forest seedlings, potentially offering a sustainable and eco-friendly method for disease management.
How do temperature, humidity, and light conditions affect the development and spread of Botrytis cinerea in vineyards?10 answersThe development and spread of Botrytis cinerea in vineyards are significantly influenced by temperature, humidity, and light conditions, as evidenced by various studies. The forecasting model for Botrytis cinerea, which is based on the relationship between relative humidity and temperature in the vine canopy, highlights the critical role these factors play in the pathogen's lifecycle. Similarly, the integration of a fungal disease forecast model into agroNET, utilizing data from connected sensors measuring air temperature, relative humidity, rain precipitation, and leaf wetness, underscores the importance of these environmental parameters in managing the disease.
Research on the interaction between tomato and Botrytis cinerea under various temperature and humidity conditions revealed that low humidity is not conducive to mycelial growth, while both high and low temperatures at high humidity can inhibit pathogenic infection. This is consistent with findings on cannabis, where Botrytis cinerea thrives under optimal relative humidity conditions (>70%) and moderate temperatures (17–24°C). The use of beneficial microorganisms, such as Paraburkholderia phytofirmans PsJN, has shown potential in controlling B. cinerea growth, indicating that direct microbial interactions can also influence disease development.
Immunological techniques developed for detecting Botrytis cinerea conidia have provided insights into the pathogen's epidemiology, further emphasizing the role of environmental conditions in its spread. Predictive models of Botrytis cinerea favorability based on meteorological data have demonstrated the significant impact of weather conditions on disease risk. Studies on biocontrol agents (BCAs) have shown that their effectiveness in controlling Botrytis bunch rot varies with temperature and humidity levels, suggesting that these environmental factors can influence the success of biological control strategies. Finally, research on disease risk indicators, including early grapevine vegetative growth and potential berry susceptibility, has highlighted the influence of weather conditions before harvest on disease development, with early NDVI and tannin content in berry skin being significant factors.
What are the mechanisms of phytoalexin detoxification by Botrytis cinerea?10 answersBotrytis cinerea, a notorious plant pathogen, employs a multifaceted approach to detoxify phytoalexins, which are antimicrobial compounds produced by plants as part of their defense mechanism. The fungus's ability to metabolize a wide range of phytoalexins, including rishitin, capsidiol, and resveratrol, is central to its virulence and broad host range. Specifically, B. cinerea can directly oxidize rishitin and capsidiol into less toxic forms through the action of specific oxidoreductases and dehydrogenases, such as Bcin08g04910, Bcin16g01490, and Bccpdh, which are upregulated in response to these phytoalexins. This indicates that the fungus has distinct mechanisms to detoxify structurally similar sesquiterpenoid phytoalexins.
Moreover, B. cinerea utilizes efflux pumps, such as BcatrB, to export a variety of structurally unrelated phytoalexins and fungicides out of the cell, preventing their accumulation to toxic levels. This efflux mechanism is supported by the induction of BcatrB expression by specific phytoalexins, demonstrating the fungus's ability to recognize and respond to different phytoalexins by activating appropriate detoxification mechanisms. Additionally, B. cinerea can degrade the broad-spectrum antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) through a combination of efflux and enzymatic degradation, involving the efflux pump BcAtrB and the extracellular laccase BcLCC2, which requires tannic acid as a mediator.
The fungus also exhibits a capacity for biotransformation, converting phytoalexins and other antifungal compounds into metabolites with diminished antifungal activity, as seen with the biotransformation of isoprobotryan-9α-ol and chloroindanol derivatives. These strategies, coupled with the upregulation of antioxidant enzymes to counteract reactive oxygen species (ROS) produced by plants, and the activation of specific virulence-related genes during infection, underscore the complex and adaptive nature of B. cinerea's mechanisms for phytoalexin detoxification.
What are the most important findings about Botrytis cinerea since 1804?5 answersBotrytis cinerea, a necrotrophic fungal plant pathogen commonly known as gray mold, has been the focus of several important findings. It has been discovered that the plant hormone cytokinin (CK) plays a dual role in the biology of B. cinerea, with its effect depending on sugar availability. CK inhibits the growth and development of B. cinerea, while also promoting glycolysis and energy consumption in the fungus. The effect of CK on B. cinerea is tied to nutrient availability, with a stronger impact in low sugar environments. Additionally, extracellular vesicles produced by B. cinerea have been identified and found to contain proteins involved in transport, metabolism, cell wall synthesis, and cell-to-cell communication. Furthermore, infection by B. cinerea induces local hypoxia in leaves, leading to the stabilization and nuclear relocalization of oxygen-unstable Ethylene Response Factors (ERF-VII). These findings provide insights into the interaction between B. cinerea and its host plants, highlighting the role of CK, energy metabolism, extracellular vesicles, and hypoxia in the pathogen's biology and virulence.
What is the role of the botrytis plant pathogens in the infection process?5 answersBotrytis plant pathogens play a crucial role in the infection process by manipulating the host and inducing cell death to facilitate colonization. These pathogens secrete proteins that actively induce plant cell death by co-opting the programmed cell death machinery of the host. The effector proteins produced by these pathogens are important pathogenic factors that can enhance their pathogenicity and are involved in various processes such as fungal sclerotium development, infection cushion formation, biosynthesis of melanin, and change in environmental pH values. The infection of plant tissues by Botrytis cinerea depends on reactive oxygen species generation and detoxification, transporter activities, plant cell wall degradation and modification, toxin production, and probable plant defense evasion by effector proteins. Additionally, the secretion of effectors by Botrytis cinerea during plant penetration, such as phytotoxins, proteases, cutinases, and plant cell death-inducing proteins, further supports their role in the infection process.