What are the standard reference values for transformation efficiency in Eschericia coli?
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The standard reference values for transformation efficiency in Escherichia coli typically range from 1 × 10^8 to 3 × 10^8 transformed colonies per microgram of plasmid DNA under standard laboratory conditions . Another method reports a transformation efficiency of approximately 5 × 10^7 transformants per microgram of plasmid DNA . Additionally, a study optimized the transformation of a malaria vaccine candidate gene into E. coli, achieving a transformation efficiency of 1.9 x 10^2 colony-forming units per microgram, using a heat shock method with specific parameters . Furthermore, a convenient and rapid method involving the use of antimicrobial peptides and specific concentrations of Ca^2+ and Mn^2+ in the transformation buffer resulted in highly competent E. coli cells with a transformation efficiency comparable to electroporation, reaching 3.1 × 10^9 colony-forming units per microgram for plasmid transformation .
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9 Citations | The transformation efficiency in Escherichia coli with plasmids using the CRM method is 3.1 ± 0.3 × 10^9 cfu/µg, comparable to electroporation, making it highly efficient for gene cloning. |
| Not addressed in the paper. | |
1 Citations | The reported transformation efficiency in Escherichia coli using the described method is approximately 5 × 10^7 transformants per microgram of plasmid DNA. |
| The optimized transformation efficiency for CIDR1α-PfEMP1 gene in Escherichia coli BL21(DE3) was 1.9 x 10^2 CFU/µg using 50 secs heat shock and 200 µL plating volume. | |
17 Citations | Transformation efficiencies in Escherichia coli using the Inoue method typically range from 1 × 10^8 to 3 × 10^8 transformed colonies per microgram of plasmid DNA under standard laboratory conditions. |
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