What is the effect of linker length on protein dimerization?
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The effect of linker length on protein dimerization varies depending on the specific system being studied. In the case of ligands targeting vascular endothelial growth factor A (VEGF), the length of the poly(ethylene glycol) (PEG) linker in homodimer peptides was found to impact binding affinity, with an optimal length resulting in a 40-fold improvement in affinity compared to a monomer control . For amyloid β (Aβ) oligomers, shorter linkers between Aβ40 monomers in dimer models were associated with stronger cytotoxicity, while longer linkers resulted in lower cooperativity and heterogeneous complexation . In the context of LC8 binding to intrinsically disordered proteins (IDPs), the spacing and sequence of motifs within the protein ASCIZ were found to control binding affinity and compositional heterogeneity of multivalent duplexes . For protein-DNA dimerizers, the length and composition of the linker were important for optimizing dimerization and binding to DNA sites . Overall, linker length plays a crucial role in protein dimerization, impacting binding affinity, cytotoxicity, complex formation, and functional optimization .
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| Papers (4) | Insight |
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| The paper does not specifically mention the effect of linker length on protein dimerization. The paper discusses the use of linkers, particularly Gly-rich linkers, to create stable protein-protein complexes and connect independent domains, but it does not provide information on the specific effect of linker length on protein dimerization. | |
11 Citations | The paper discusses the importance of linker length and composition in protein-DNA dimerizers, but it does not specifically mention the effect of linker length on protein dimerization. |
| The effect of linker length on protein dimerization is discussed in the paper. Short linkers result in stable duplexes but form off-register structures at high protein concentrations, while long linkers lead to lower cooperativity and heterogeneous complexation at low protein concentrations. | |
| The effect of linker length on protein dimerization is qualitatively correlated to a theoretical model in the paper. |
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