What is the process used to induce senescence in epidermal cells?
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Inducing senescence in epidermal cells involves the activation of p14^ARF, which leads to apoptosis, cell-cycle arrest, and subsequent p53-dependent senescence . This process requires continued expression of p14^ARF to commit cells to senescence, indicating a dependency on a persistent signal . Senescent cells formed in the epidermis are retained for extended periods even after transgene silencing, suggesting a lack of efficient removal mechanisms . Interestingly, epidermal hyperplasia induction can prevent the appearance of senescent cells upon p14^ARF activation . Understanding the dynamics of senescence induction in epidermal cells is crucial due to its implications in aging, tumor suppression, and tissue function .
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| Oxidative stress induced by hydrogen peroxide was used to induce senescence in retinal pigment epithelial cells in the study, inhibiting cell proliferation and increasing senescence-associated beta-galactosidase staining. | |
53 Citations | Oxidative stress leading to single-strand breaks and down-regulation of PARP1 induces senescence in epidermal cells, activating a p38MAPK-P16-RB cell cycle arrest pathway as an adaptive response. |
| Genetic and epigenetic factors induce senescence in epidermal cells through processes like replicative exhaustion, DNA damage, and metabolic dysfunction, leading to a senescent state in aged skin. | |
| p14ARF induction in the epidermis triggers apoptosis, cell-cycle arrest, and subsequent p53-dependent senescence, activating p19ARF and p16Ink4a through a feed-forward loop, requiring persistent p14ARF expression for senescence commitment. | |
| Senescence in epidermal cells was induced by transgenic p14ARF expression, leading to apoptosis, cell-cycle arrest, and subsequent p53-dependent senescence, revealing a feed-forward loop involving p19ARF and p16Ink4a. |
Related Questions
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