scispace - formally typeset
Search or ask a question

Showing papers on "Anisakis published in 2002"


Journal ArticleDOI
TL;DR: The present findings represent the first established records of intermediate/paratenic hosts of Anisakis typica and extend its range to Somali waters of the Indian Ocean and to the Eastern Mediterranean Sea.
Abstract: Genetic variation at 21 gene-enzyme systems was studied in a sample of an adult population of Anisakis typica (Diesing, 1860) recovered in the dolphin Sotalia fluviatilis from the Atlantic coast of Brazil. The characteristic alleles, detected in this population, made it possible to identify as A. typica, Anisakis larvae with a Type I morphology (sensu Berland, 1961) from various fishes: Thunnus thynnus and Auxis thazard from Brazil waters, Trachurus picturatus and Scomber japonicus from Madeiran waters, Scomberomorus commerson, Euthynnus affinis, Sarda orientalis and Coryphaena hippurus from the Somali coast of the Indian Ocean, and Merluccius merluccius from the Eastern Mediterranean. Characteristic allozymes are given for the identification, at any life-stage and in both sexes, of A. typica and the other Anisakis species so far studied genetically. The distribution of A. typica in warmer temperate and tropical waters is confirmed; the definitive hosts so far identified for this species belong to delphinids, phocoenids and pontoporids. The present findings represent the first established records of intermediate/paratenic hosts of A. typica and extend its range to Somali waters of the Indian Ocean and to the Eastern Mediterranean Sea. A remarkable genetic homogeneity was observed in larval and adult samples of A. typica despite their different geographical origin; interpopulation genetic distances were low, ranging from D(Nei)=0.004 (Eastern Mediterranean versus Somali) to D(Nei)=0.010 (Brazilian versus Somali). Accordingly, indirect estimates of gene flow gave a rather high average value of Nm = 6.00. Genetic divergence of A. typica was, on average, D(Nei)=1.12 from the members of the A. simplex complex (A. simplex s.s, A. pegreffii, A. simplex C) and D(Nei)=1.41 from A. ziphidarum, which all share Type I larvae; higher values were found from both A. physeteris (D(Nei)=2.77)

119 citations


Journal ArticleDOI
TL;DR: Results indicate that high-pressure treatment is an alternative nonthermal method for killing this nematode, and uses for this treatment in food processing are suggested.

66 citations


Journal ArticleDOI
TL;DR: It is proposed that the PCR-RFLP assay described in this report may be used for identification of marine and freshwater parasites from superfamily Ascaridoidea.

46 citations


Journal ArticleDOI
TL;DR: It is indicated that the activation of the fish immune system by infection with live anisakids influences the skin response of the host with a subsequent negative effect on the ectoparasites G. derjavini.

31 citations


Journal ArticleDOI
TL;DR: IgE responses to Anisakis were also observed in habitual consumers of raw fish, without any clinical manifestations, suggesting that the relevance of results of conventional tests has to be interpreted on the basis of clinical aspects.
Abstract: Anisakis simplex is a seafish nematode, which is responsible for the well-known human infection (anisakiasis) and can induce IgE-mediated reactions. IgE sensitization to Anisakis simplex can be frequent in particular countries and should be suspected in patients with acute allergic symptoms after ingestion of fish. The etiological role of Anisakis simplex was evaluated in 49 adult subjects with acute allergic symptoms after ingestion of anchovies. Serum-specific IgE and prick tests to anchovy were negative in each patient. Specific IgE reactions to Anisakis were positive in 45 patients and skin tests in 43. Only 3 patients with allergy to the nematode were atopic. However, IgE responses to Anisakis were also observed in habitual consumers of raw fish, without any clinical manifestations, suggesting that the relevance of results of conventional tests has to be interpreted on the basis of clinical aspects.

28 citations


Journal ArticleDOI
TL;DR: A female patient with no personal or family history of allergy who experienced two episodes of anaphylactic shock who developed generalized urticaria provoked by crustacean (prawns) and white fish is reported.
Abstract: Because seafood consumption is moderate-to-high in Spain, allergic reactions to seafood such as fish, crustacea and mollusc are fairly frequent. The clinical features of these reactions depend on the implicated species and whether the reaction is provoked by ingestion, handling or vapor inhalation. Because different species have common antigenic structures, cross-sensitization is frequent, especially between crustaceans and molluscs. Contamination of fish by nematodes (Anisakis) may produce severe reactions. We report the case of a female patient with no personal or family history of allergy who experienced two episodes of anaphylactic shock: the first occurred immediately after eating oysters and the second after ingestion of white fish. The patient also developed generalized urticaria provoked by crustacean (prawns) and white fish. The results of skin prick tests were negative for fish, shellfish, crustacean and oysters while in vitro tests were positive for oyster, prawns, Anisakis, Ascaris and Echinococcus, although stool samples and gastric endoscopy were negative.

19 citations


Journal Article
TL;DR: The possible migration of nematodes from the intestines into the muscle of ungutted saithe, haddock and ocean perch was studied and Anisakis larvae are present in the flesh of all three species already at capture, but no post-mortem migration into the flesh was observed during ice storage.
Abstract: The possible migration of nematodes from the intestines into the muscle of ungutted saithe, haddock and ocean perch was studied. Fishes were examined immediately after catch and after ice storage for 6 days or longer. The results have shown that Anisakis larvae are present in the flesh of all three fish species already at capture, but no post-mortem migration into the flesh was observed during ice storage. Abundance and prevalence varied between the three fish species. Studies on the occurrence of nematodes in pike-perch revealed that fishes from the Greifswalder Bodden were infected by nematodes, both in muscle and intestines. The infection rate increased with fish size and was related to the fishing month.

15 citations


Journal ArticleDOI
01 Jul 2002
TL;DR: In this article, two probable cases of acute gastric anisakisis by eating "cebiche" of "perico" have been founded; the prevalence of Anisakis physeteris larvae was 7/12 and relative intensity was of 14-63 (X=34.28) larvae.
Abstract: In this report two probable cases of anisakiosis and results on search of Anisakis larvae in 12 old specimens of Coryphaena hippurus "perico" or "dorado" that were expended at the markets of Ica city from Marcona and San Andres (Department of Ica) and Ilo (Department of Moquegua) in Central and South peruvian coast during phenomenon "El Nino" 1997-98 are presented. Two probable cases of acute gastric anisakisis by eating "cebiche" of "perico" have been founded; the prevalence of Anisakis physeteris larvae was 7/12 and relative intensity was of 14-63 (X=34.28) larvae. It's thought that anisakiosis emerges during phenomenon El Nino and some measure of controls in order to low the risk of infection by this marine zoonoses are proposed

14 citations


Journal ArticleDOI
TL;DR: This preliminary investigation contrasts sharply with previous studies in the native range of American shad and confirms that this fish may be an important intermediate host for A. simplex in the Pacific Northwest.
Abstract: This paper represents the first report of the nematode Anisakis simplex in the American shad (Alosa sapidissima) in its introduced range in the American Pacific Northwest. All the adult shad sampled from spawning populations in the Willamette (n = 9) and Umpqua (n = 12) rivers were infected with A. simplex with intensities ranging from 6 to 89 worms per fish. This preliminary investigation contrasts sharply with previous studies in the native range of American shad and confirms that this fish may be an important intermediate host for A. simplex in the Pacific Northwest. It is suggested that this new parasite-host relationship has led to an ecological expansion into rivers and Anisakis may present an emerging health risk for wildlife and some human consumers.

14 citations


Journal ArticleDOI
01 Mar 2002-Sarsia
TL;DR: Very small larvae acquired by M. muelleri are able to establish in its visceral cavity, and larger larvae do not mature in this host, which is clearly an unsuitable final host to this parasite.
Abstract: Maurolicus muelleri collected at 70-220 m in Herdlefjorden, W Norway, were examined for metazoan parasites A total of seven species were found: Derogenes varicus and Brachyphallus crenatus (Trematoda), tetraphyllidean larvae and Bothriocephalus sp (Cestoda), Hysterothylacium aduncum and Anisakis simplex larvae (Nematoda) and Sarcotretes scopeli (Copepoda) The parasite community was completely dominated by larval helminths (9989%) Of 3,720 parasites found, only 5 specimens (4 trematodes and 1 copepod) were adults Of the helminth larvae, 994% were Hysterothylacium aduncum The infracommunities were species-poor; 986% contained 0 or 1 species only; and diversity was low (mean Brillouin's index = 030) The H aduncum larval size spectrum in age groups of M muelleri is described, and the following observations are made: very small larvae ( 3 mm) do not mature in this host This is at variance with other s

6 citations


Journal Article
TL;DR: The Authors report, for the first time, the presence of an Anisakis larva in one specimen of Sepia officinalis, describing the characteristics of parasitic localization coexisting with an Aggregata sp.
Abstract: The Authors report, for the first time, the presence of an Anisakis larva in one specimen of Sepia officinalis, describing the characteristics of parasitic localization coexisting, moreover, with an Aggregata sp. infection. The AA conclude with some sanitary and inspective considerations.

Journal Article
TL;DR: The hight parasitization rate of Anisakis simplex larve in fishes warns us against the great risk of anisakiasis by eating raw fishes.
Abstract: An investigation of Anisakis simplex larvae,the most important causative agent of anisakiasis,in marine fishes caught in Bohai sea was carried out in order to detect their infectivity.METHOD:the fishes were dissected to detect Anisakis larvae.The collected larvae were fixed with 70% efhanol solution and cleared as usual with a 70% ethanol/glycerine mixture.the morphological feature of the larve were observed and indentified microscopically.RESULTS:Among all species examed ,26 out of 49 species(53.1%) were posivie for Anisakis simplex larve in Bohai sea. The infection rates of 26 positive species in the former were 47.2%(83/176).CONCLUSION:the hight parasitization rate of Anisakis simplex larve in fishes warns us against the great risk of anisakiasis by eating raw fishes.