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Showing papers on "Brilliant green published in 1987"


Journal ArticleDOI
TL;DR: Results suggested that anaerobically exposed Escherichia coli B cells may serve as an agent which renders E. coli cells susceptible to the basic dyes.
Abstract: Phosphate buffer suspensions of resting Escherichia coli B cells at pH 7.0 were anaerobically exposed to alternating current (a.c.) of 50 Hz at a current density of 600 +/- 60 mA/cm2 and 34 degrees +/- 3 degrees C. The minimum inhibitory concentrations of eight basic dyes: crystal violet, malachite green, brilliant green, fuchsin, methylene blue, toluidine blue, safranin and acriflavine for exposed cells were decreased to about the half values of those for unexposed ones when both cells were grown in the minimal medium including one of the dyes. The integrated viabilities of exposed cells tended to decline with increasing concentration of the dyes markedly more than those of unexposed ones, whereas the exposed cells took up the dyes less readily than the unexposed cells. These results suggested that a.c. exposure may serve as an agent which renders E. coli cells susceptible to the basic dyes.

14 citations


Journal ArticleDOI
TL;DR: In this paper, the effects of pH and diverse ions on perchlorate spectrophotometrically at 639 nm after its adsorptive extraction with Brilliant Green on microcrystalline benzophenone at pH 6.5 after dissolution of the solid phase in benzene.

13 citations


Journal ArticleDOI
TL;DR: In this article, the suitability of stained food and [ 103 Ru]phenanthroline-treated food as markers for measuring dietary retention time was examined in sheep, and the mean retention time estimated with the stain was about 40% longer than that estimated with [ 103Ru]-phenanthyline.
Abstract: SUMMARY The suitability of stained food and [ 103 Ru]phenanthroline-treated food as markers for measuring dietary retention time was examined in sheep. The digestibility of oat husk, assessed by incubation of samples contained in nylon bags in the rumen, was greatly depressed by staining with brilliant green, and slightly depressed by basic fuschsin. The latter effect appeared largely to be due to boiling rather than the stain itself. When milled hay treated with both basic fuschsin and [ 103 Ru]phenanthroline was introduced into the rumen, the mean retention time estimated with the stain was about 40% longer than that estimated with [ 103 Ru]phenanthroline.

7 citations


Journal ArticleDOI
TL;DR: The suitability and selectivity of nutrient broth with bile salts and varying concentrations of brilliant green as an enrichment medium was tested for the isolation of Plesiomonas shigelloides from faeces, finding it most effective at a concentration of 0.001 g/l brilliant green.
Abstract: The suitability and selectivity of nutrient broth with bile salts and varying concentrations of brilliant green as an enrichment medium was tested for the isolation of Plesiomonas shigelloides from faeces. The broth was most effective at a concentration of 0.001 g/l brilliant green, using faeces deliberately contaminated with Plesiomonas. Two hundred and nineteen faecal samples from Nigeria were then examined for the presence of Plesiomonas and other bacterial pathogens. One yielded Plesiomonas following enrichment in bile salts brilliant green broth (BBG broth) whereas no samples were positive on direct plating.

4 citations


Journal Article
TL;DR: Brilliant green was examined as a detection reagent in the separation of some aliphatic alcohols, ketones and esters by the reverse-phase liquid chromatography as discussed by the authors.
Abstract: Brilliant green was examined as a detection reagent in the separation of some aliphatic alcohols, ketones and esters by the reverse-phase liquid chromatography. These samples not possessing chromophore could be indirectly detected by photometric detector with the assistance of brilliant green in visible range (625nm). Some mixtures of samples were also able to separated and detected with good base line resolution and comparatively high sensitivity.

1 citations