Showing papers on "Bufo melanostictus published in 2005"

Quality evaluation of traditional Chinese drug toad venom from different origins through a simultaneous determination of bufogenins and indole alkaloids by HPLC.

Abstract: A simple and efficient HPLC method has been developed to evaluate the quality of traditional Chinese medicine toad venoms. The major bioactive ingredients, including 10 bufogenins and 4 indole alkaloids in the drug, were separated and quantified on a phenyl-hexyl column with a UV detector. A total of 27 toad venom samples from two species, Bufo bufo gargarizans CANTOR and Bufo melanostictus SCHNEIDER, from the different drug production regions of China, were analyzed. The chromatograms showed significant differences with respect to the samples from different origins. These toad venom samples can be distinctly classified into 4 groups by cluster analysis using the contents of the 14 main constituents, including toad venom samples from B. bufo gargarizans from north China, median China and south China, and samples from B. melanostictus from south China. Toad venom samples from B. bufo gargarizans from median China were considered to be of the highest quality.

Effects of prolonged administration of large doses of acth on bufo melanostictus tadpoles

Abstract: Field ponds usually contain adpoles of severalt simultaneous hatchings; collection of such animals indicate variability in the standards of experimental parameters. In order to avoid such variations, numerous Bufo melanostictus tadpoles belonging to single hatchings were collected separately, from a variety of ponds at the University Campus. ceased its wriggling movements; the Petri dish was After bringing them to the laboratory, the tadpoles were divided into suitable groups and were given refined suspension of parboiled spinach.

Immunohistochemical Study on 5-Hydroxytryptamine Immunoreactive Cells in the Alimentary Tract of Bufo melanostictus and Rana nigromaculata

Abstract: Using immunohistochemical technique, 5-Hydroxytryptamine immunoreactive (5-HT-IR) cells were observed in the alimentary tract of the toad Bufo melanostictus and the frog Rana nigromaculata . 5-HT-IR cells were distributed throughout the alimentary tracts of both species and located in the gastric glands of the stomach regions and in the intestinal or esophageal epithelium with variable frequencies. The density of the cell in B. melanostictus is the highest in the ileum, moderate in the jejunum and stomachus pyloricus, and the lowest in the duodenum and rectum. In R. nigromaculata the density of 5-HT-IR cells is the highest in the stomachus pyloricus, moderate in the ileum and jejunum, and the lowest in the esophagus, stomachus cardiacus and rectum. 5-HT-IR cells were spherical, elliptoid, coniform or spindle-shaped with long cytoplasmic process reaching to the lumen. The distribution mode of 5-HT-IR cells and the relationship between morphology and function are also discussed.