Showing papers on "Gentiana published in 2004"
TL;DR: In this article, Gentiana macrophylla (Gentianaceae) extract was administered to rheumatoid rats at a daily dose of 100 mg/kg body weight, and the prostaglandin E(2) (PGE(2)) levels in the inflammatory tissues, sole thickness, and ankle circumferences of feet were significantly decreased.
68 citations
TL;DR: Micellar electrokinetic electrophoresis was employed to determine two active components, gentiopicroside (GE) and swertiamarin (SW) in one Tibetan preparation medicine named shiweilongdankeli, two Tibetan herbal medicines named Gentiana rhodantha and Gentiana kitag and three other Chinese Gentiana medicines.
Abstract: Micellar electrokinetic electrophoresis was employed to determine two active components, gentiopicroside (GE) and swertiamarin (SW) in one Tibetan preparation medicine named shiweilongdankeli, two Tibetan herbal medicines named Gentiana rhodantha and Gentiana kitag and three other Chinese Gentiana medicines named Gentiana scabra, Gentiana rigescens and Gentiana macrophylla. The dissociation constants of gentiopicroside and swertiamarin determined by MEKC were 7.71 and 6.25. The optimum buffer system was 70 mm borate-10 mm sodium dodecylsulfate (SDS) -6% (v/v) ispropanol (pH 9.0). The voltage was 15 kV and detection was at 254 run. The lower limits of detection (defined as a signal-to-noise ratio of about 3) were approximately 3.86 mg L-1 for GE and 5.88 mg L-1 for SW. The relative standard deviation of the migration time and peak area of the GE and SW were 2.33, 2.47 and 1.27, 2.19%, respectively and the recoveries of the two compounds were 96-104% for GE and 92-102% for SW. Copyright (C) 2004 John Wiley Sons, Ltd.
21 citations
TL;DR: The aim of this work was to produce interspecific hybrids between G. triflora and G. lutea with the ultimate aim being to produce yellow‐flowered cultivars.
Abstract: Gentiana is increasingly being exploited as a cut flower crop but its colour range in commercial varieties is limited despite a wide range of colours being present in the genus. The aim of this work was to produce interspecific hybrids between G. triflora and G. lutea with the ultimate aim being to produce yellow‐flowered cultivars. Six to 8‐day‐old embryos were rescued from the G. triflora parent and cultured within their ovules on modified B5 medium. Following embryo germination, plantlets were transferred to modified Murashige & Skoog (MS) medium for further growth and proliferation. Shoots were then transferred to growth‐regulator free, modified MS medium for root initiation before transfer to the greenhouse. However, plants died within a few months of transfer to the greenhouse. The hybrid nature of the embryo‐derived plants was indicated first by flow cytometry, then confirmed by chromosome counts and examination of morphological features. Mitotic chromosome counts of one of the G. triflora...
13 citations
Journal Article•
TL;DR: In this paper, four compounds were isolated from the ethanol extracts of Gentiana apiata N.E.Br. firstly.On the basis of spectral analysis, these compounds were elucidated as isoorientin (�),gentiopicroside (Ⅱ), apiatoside A(Ⅲ) and D-(+)-sucrose() of which Ⅲ is a new compound.
Abstract: Four compounds were isolated from the ethanol extracts of Gentiana apiata N.E.Br. firstly.On the basis of spectral analysis,these compounds were elucidated as isoorientin (Ⅰ),gentiopicroside (Ⅱ), apiatoside A (Ⅲ) and D-(+)-sucrose(Ⅳ),of which Ⅲ is a new compound.
3 citations
Journal Article•
TL;DR: The results show that the content of each trace element is different in each part of Gentiana straminea Maxim, and in the part between stem and root its trace elements content is much higher than the other parts, and the content in root is the lowest.
Abstract: Gentiana straminea Maxim.is one of the famous Tibetan medicines.The content of four trace elements As,Hg,Pb and Cr in it was determined.The results show that the content of each trace element is different in each part of Gentiana straminea Maxim.Especially in the part between stem and root its trace elements content is much higher than the other parts,and the content in root is the lowest.This study can be considered as a base work for the further research on the Tibetan medicine.
1 citations