Showing papers on "Karanjin published in 2012"

Oil, Fatty Acid Profile and Karanjin Content in Developing Pongamia pinnata (L.) Pierre Seeds

Abstract: Oil content, fatty acid composition and karanjin content were studied in developing pongamia seeds, at intervals of 3 weeks from 30 weeks after flowering up to 42 weeks Three marked stages in seed development were observed at the early green pod stage, the middle half brown stage and the late dark brown stage Significant variation in seed biomass, pod and seed characteristics were observed A significant (P < 001) decrease in the moisture content of the seeds was observed during seed development The oil content gradually increased from 3206 to 3653 % as the seed matured A significant variation in fatty acid composition was detected across all stages of seed development Palmitic acid (16:0) content marginally decreased from 1181 to 1018 %, while stearic acid (18:0) and linolenic acid (18:3) remained constant at all stages of seed maturity A steady increase in oleic acid (18:1) content from 3811 to 4911 % was observed, while the linoleic acid (18:2) content decreased from 3014 to 1885 % The iodine value increased, while the saponification number of oil decreased during seed development The increase in karanjin content was steady Seeds harvested after 42 week after flowering yielded the maximum oil with high oleic acid content which could be suitable for biodiesel production

A simple and improved method for isolation of karanjin from Pongamia pinnata Linn. seed oil

Abstract: 1 Study was undertaken with the objective to develop a simple method for isolation of karanjin from Karanja (Pongamia pinnata Linn.) seed oil. The seed oil was subjected to liquid-liquid extraction with methanol. The extract was further subjected to solvent partitioning followed by crystallization to get karanjin, purity of which was ascertained by HPLC. The purity of isolated karanjin was found to be 98%. Mass spectrum for the compound in ESI + mode showed signals at 293 (M+H) + , which confirmed the molecular weight to be 292. From IR, 1 H and 13 C NMR spectral data, structure elucidation was done and the structure was conformed as karanjin.

Development and Validation of an HPLC Method for Karanjin in Pongamia pinnata linn. Leaves.

Abstract: A rapid, simple and specific reversed-phase HPLC method has been developed for analysis of karanjin in Pongamia pinnata Linn. leaves. HPLC analysis was performed on a C18 column using an 85:13.5:1.5 (v/v) mixtures of methanol, water and acetic acid as isocratic mobile phase at a flow rate of 1 ml/min. UV detection was at 300 nm. The method was validated for accuracy, precision, linearity, specificity. Validation revealed the method is specific, accurate, precise, reliable and reproducible. Good linear correlation coefficients (r2>0.997) were obtained for calibration plots in the ranges tested. Limit of detection was 4.35 μg and limit of quantification was 16.56 μg. Intra and inter-day RSD of retention times and peak areas was less than 1.24% and recovery was between 95.05 and 101.05%. The established HPLC method is appropriate enabling efficient quantitative analysis of karanjin in Pongamia pinnata leaves.

A method for isolation of karanjin from the expelled cake of Pongamia glabra

Abstract: Due to the renewed interest in the production of biodiesel from nonconventional oils like karanja (Pongamia glabra), huge quantity of expelled cake will be generated in near future. However, due to the presence of several antinutritional components, expelled karanja cake cannot be used as feed for poultry and livestock. It needs detoxification and the first step during detoxification is the removal of karanjin, the major bioactive constituent. The present study aimed at isolation of karanjin from expelled cake. Accordingly, a simple and scalable process was developed for the isolation of karanjin with purity in the range of 95–97% from the expelled cake of karanja. Different solvents were screened by varying temperature, time, and amount of extracting solvents to extract karanjin from the expelled cake and dimethyl carbonate (DMC) was found to be the best among them. DMC extraction of 1.0 kg of expelled cake yielded 3.6 g (0.36% with respect to expelled cake) of karanjin of 97% purity.

Method for purifying high-content karanjin

Abstract: The invention relates to a method for purifying high-content karanjin, comprising the following steps: crushing cauline branches of fordia cauliflora hemsl, extracting and concentrating with ethanol having a certain concentration, extracting twice with petroleum ether, using macroporous resin on the extract, concentrating the eluent and separating through a polyamide column, and recrystillizing through 95 % ethanol. The method has the advantages of low production cost and simplicity, and is easy to batch production. The cauline branches are used as raw material, thus the invention has good resource advantage.

Development and Validation of a HPTLC method for determination of Karanjin in Pongamia pinnata: A novel Indian medicinal plant

Abstract: Pongamia pinnata is a popular Indian medicinal plant used for treatment of bronchitis, whooping cough, rheumatic arthritis, and diabetes diseases. Karanjin in one of the prevalent phytoconstituent present in this plant. A sensitive, selective and precise thin-layer chromatographic method has been developed and validated for the analysis of Karanjin in Pongamia pinnata. Separation and quantification was achieved by TLC using mobile phase of ethanolethyl acetatate (7:3, v/v), (RF 0.69) on precoated silica gel 60F254 aluminium plates and densitometric determination was carried out in reflection/absorption mode at 260 nm. The calibration curve was linear in the concentration range of 0.1μg-1.2μg spot-1. The method was validated for precision, repeatability and accuracy. The proposed method was found to be simple, precise, specific, sensitive and accurate for the quantification of Karanjin.