Showing papers on "Mitochondrial DNA replication published in 1978"
TL;DR: Pulse-chase radioactive labeling experiments using thymidine kinase-plus mouse LA9 cells have shown that the 7 S mitochondrial DNA initiation sequence of mitochondrial DNA is synthesized and turned over at a faster rate than previously determined, indicating that the extent of 7S mitochondrial DNA synthesis is most likely determined by a nucleotide sequence specific event.
107 citations
TL;DR: Nine transcripts complementary to mouse L cell mitochondrial DNA have been detected, sized and mapped to restriction fragments using the method of Berk and Sharp (1977), indicating that all stable transcripts 300 bases or longer detected by this technique originate from genes on the H strand.
72 citations
TL;DR: The degree of sensitivity to alkali has been used to demonstrate that newly replicated mitochondrial DNA has the same content of ribonucleotides as mass-labeled mitochondrial DNA.
40 citations
TL;DR: The DNA of several strains of Saccharomyces cerevisiae was labeled and followed for three generations after transfer of cultures from light medium to heavy medium and heavy medium to light medium, and the density shifts observed for mitochondrial DNA were those expected for semiconservative, nondispersive replication.
Abstract: The DNA of several strains of Saccharomyces cerevisiae was labeled by growing the culture in medium supplemented with thymidylate and bromodeoxyuridylate. It was thus possible to follow the course of mitochondrial DNA replication in density shift experiments by determining the buoyant density distribution of unreplicated and replicated DNAs in analytical CsCl gradients. DNA replication was followed for three generations after transfer of cultures from light medium to heavy medium and heavy medium to light medium. Under both conditions, the density shifts observed for mitochondrial DNA were those expected for semiconservative, nondispersive replication. This was further confirmed by analysis of the buoyant density of alkali-denatured hybrid mitochondrial DNA. With this method, no significant recombination between replicated and unreplicated DNA was detected after three generations of growth.
9 citations