Showing papers on "Murashige and Skoog medium published in 1969"
TL;DR: Geranium callus produced from explants of stem tip, internode pith with vascular tissute on synthetic media with or without coconut milk and 2,4-dichlorophenoxyacetic acid grew well for many generations, but only tracheids were induced in them.
Abstract: A B S T R A C T Geranium callus produced from explants of stem tip, internode pith with vascular tissute on synthetic media with or without coconut milk and 2,4-dichlorophenoxyacetic acid grew well for many generations, but only tracheids were induced in them. If callus produced on these media was subcultured immediately on Murashige and Skoog medium with 0.1 mg/liter/a-naphthalene acetic acid (NAA) and 10.0 mg/liter kinetin (K) and incubated at 16/8-hr light/dark cycle, shoots were induced in 8-10 weeks and roots in another 8-10 weeks. Callus produced on the MS medium with the same supplements of NAA and K, subcultured on the same medium and incubated in 16/8-hr light/dark cycle, produced shoots in 6-8 weeks. However, on callus subcultured more than three times, shoots differentiated with greater difficulty and none differentiated after six subeultures. Some abnormal shoot-like structures were also produced, the cells of which showed virus-like inclusion bodies. Requirements of the different varieties for differentiating organs differed. Among 12/12-, 1a/9-, 16/8-, and 20/4-hr light/dark photoperiods that induced differentiation, 15/9- and 16/8-hr were more effective than the others. Continuous illumination did not induce differentiation. Differentiated shoots formed roots more readily on a medium with reversed proportions of auxin and kinetin. On agar roots were devoid of root hairs. Root hairs were formed when the shoots and plantlets were cultured on filter-paper bridges. Many "mother" stock plants were produced. These are being studied for their growth qualities and for possible viruses and other pathogens.
57 citations
TL;DR: Tumor cultures grew well on a minimal medium lacking kinetin, indoleacetic acid, vitamins, glycine, and inositol, and Glycine was necessary only in the growth of N. langsdorffii pith callus.
Abstract: Explants of genetic tumors, tumors initiated by Agrobacterium tumefaciens strains B-6 and T-37, and excised pith plugs from Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii were cultured on Murashige and Skoog medium. All cultures, pith callus and tumors with the exception of N. langsdorffii pith grew on this medium. Addition of glutamine to the medium resulted in highly organoid growth in N. langsdorffii pith. In order to have material comparable to other pith cultures, N. langsdorffii was initiated on 2,4-dichlorophenoxyacetic acid medium, after which it grows on complete medium as amorphous pith callus. Except for the initiation of N. langsdorffii (and N. glauca) pith, the 2,4-dichlorophenoxyacetic acid medium, caused bleaching in cultures of T-37 induced tumors and death of B-6 induced tumors. Tumor cultures, except for the seedling tumor, grew well on a minimal medium lacking kinetin, indoleacetic acid, vitamins, glycine, and inositol. Glycine was necessary only in the growth of N. langsdorffii pith callus. A tissue culture model is presented which permits comparison of the various tissue types.
8 citations
TL;DR: Rapid propagation of yam was obtained from nodal segments cultured in modified Murashige and Skoog medium containing indoleacetic Acid and kinetin as establishment medium, and naphthaleneacetic acid (NAA) as rooting medium.
Abstract: Rapid propagation of yam ( Dioscorea rotundata cv. Habanero) was obtained from nodal segments cultured in modified Murashige and Skoog medium containing indoleacetic acid (IAA) and kinetin as establishment medium, and naphthaleneacetic acid (NAA) as rooting medium. The proliferation cycle, which takes approximately 2-3 months, increased four times the production of yam plantlets. These plantlets were successfully transferred to potting mixture and soil. This procedure is extremely useful for regenerating virusfree plantlets suitable for producing healthy tubers for planting.
4 citations
TL;DR: Young leaves of Liriope muscari provide an ample source of explants for in vitro propagation in tropical countries where flowering is scarce, but in variegated varieties, only green or white plants were produced, because of a chimera in the original tissue.
Abstract: Young leaves of Liriope muscari provide an ample source of explants for in vitro propagation in tropical countries where flowering is scarce. Leaves were induced to form calli on a solid medium containing Murashige and Skoog (MS) salts and vitamins, 3% sucrose, 0.7% agar, 1 mg/L 2,4-dichlorophenoxy- acetic acid (2, 4-D) and 1 mg/L 6-furfurylaminopurine (kinetin). Only the proximal segments of the leaves produced calli. These calli were induced to produce multiple plantlets on MS medium, 3% sucrose, 0.7% agar, and 10 mg/L N 6 (2-isopentenyl) adenine (2 ip). It is possible to use leaf explants for in vitro mass production of Liriope . However, in variegated varieties, only green or white plants were produced, because of a chimera in the original tissue.
1 citations