Showing papers on "Sodium propionate published in 2012"

Enhancing the 3-hydroxyvalerate component in bioplastic PHBV production by Cupriavidus necator

Abstract: In the current context of global warming, the substitution of conventional plastics with bioplastics is a challenge. To take up this challenge, we must meet different technical and economic constraints. In the case of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), the technical properties can be modulated by varying the 3-hydroxyvalerate content. 3-Hydroxyvalerate (3-HV) enhancement is an issue; therefore, simultaneous evaluation of several 3-hydroxyvalerate-enhancing substrates through fractional factorial design of experiments is described. Eight substrates citric, valeric, propionic, and levulinic acids; propanol; pentanol; and sodium propionate were studied for 3-HV enhancement, and sodium glutamate was studied for biomass and polyhydroxyalkanoate (PHA) enhancement. The most efficient 3-hydroxyvalerate-enhancing factors were levulinic acid, sodium propionate, and pentanol; however, pentanol, at a concentration of 1 g/L, had an extremely negative influence on biomass production and the PHA content of cells. The effect of the inoculum nutrient composition on the final 3-HVcontent was also evaluated. These results showed that the most efficient combination for the production of high 3-HVcontent in PHBV was primary inoculum growth on mineral medium followed by fermentation for 48 h with levulinic acid and sodium propionate (at 1 g/L) as the only carbon sources. This allowed us to produce PHBV with a 3-HVcontent of 80 mol % and overall volumetric and specific productivities of 2 mg/L/h and 3.9 mg/g(CDW) /h, respectively, with the addition of only 2 g/L of inducing substances.

Propionate absorbed from the colon acts as gluconeogenic substrate in a strict carnivore, the domestic cat (Felis catus).

Abstract: In six normal-weight and six obese cats, the metabolic effect of propionate absorbed from the colon was assessed. Two colonic infusions were tested in a crossover design with intervals of 4 weeks. The test solution contained 4 mmol sodium propionate per kg ideal body weight in a 0.2% NaCl solution. Normal saline was given as control solution. Solutions were infused into the hindgut over 30 min. Blood samples were obtained prior to and at various time points after starting the infusion. As body condition did not affect evaluated parameters, all data were pooled. Plasma glucose concentrations showed differences neither over time nor during or after infusion with propionate or control. Plasma amino acid concentrations rose over time (p < 0.001), but were similar for both infusions. Plasma propionylcarnitine rose markedly towards the end of the propionate infusion and decreased afterwards (p < 0.001), whereas 3-hydroxy-3-methylglutarylcarnitine was lower 30 (p = 0.005) and 60 min (p = 0.032) after ending propionate infusions and acetylcarnitine tended to fall at the same time points (p = 0.079; p = 0.080), suggesting inhibition of gluconeogenesis from pyruvate and amino acids, but initiation of propionate-induced gluconeogenesis. In conclusion, propionate absorbed from the colon is hypothesized to act as gluconeogenic substrate, regardless of the cat's body condition.

Possibilities of increasing the residual water‐soluble carbohydrate concentration and aerobic stability of low dry‐matter perennial ryegrass silage through additive and cultivar use

Abstract: Increasing the residual water-soluble carbohydrate (WSC) concentration in silage may improve the nutritional value but impair aerobic stability. Our aim was to determine whether the residual WSC concentration and aerobic stability of low dry-matter (<135 g kg−1) perennial ryegrass silage could be manipulated through the judicious use of additive and cultivar. Seven additive treatments, including three innovative treatments, were compared across four consecutive harvests of the cultivars AberDart (bred to accumulate high concentrations of herbage WSC) and Fennema (control). The standard of fermentation of silage ensiled without additive (untreated) ranged from very bad to excellent. Application of ammonium tetraformate, at 3 and 6 L t−1, or homofermentative lactic acid bacteria (LAB) alone had an inconsistent effect on the fermentation and aerobic stability, and negligible effect on residual WSC concentration. A mixture of Lactobacillus buchneri and homofermentative LAB was not an effective silage additive, producing generally poorly fermented silage. An antimicrobial mixture of sodium benzoate, sodium propionate, sodium nitrite and hexamethylenetetramine, applied at 2·5 and 5 L t−1, frequently improved the standard of fermentation, but the effects were subject to the application rate. The high application rate was the most effective additive evaluated at improving the fermentation and increasing residual WSC concentration and consistently produced silage of excellent standard of fermentation. However, the antimicrobial mixture was not effective at protecting against aerobic instability. The effects of additive treatment were largely inconsistent across cultivars. Overall, AberDart had a negligible effect on the silage fermentation, residual WSC concentration and aerobic stability compared with Fennema.

Effect of Different Biosynthetic Precursors on the Production of Nargenicin A1 from Metabolically Engineered Nocardia sp. CS682

Abstract: Nargenicin A1 is a 28-membered polyketide macrolide, with antibacterial activity against methicillin-resistant Staphylococcus aureus, produced by Nocardia sp. CS682. In this study, the production of nargenicin A1 was improved by enhancing the supply of different biosynthetic precursors. In Nocardia sp. CS682 (KCTC11297BP), this improvement was ~4.62-fold with the supplementation of 30 mM methyl oleate, 4.25-fold with supplementation of 15 mM sodium propionate, and 2.81-fold with supplementation of 15 mM sodium acetate. In Nocardia sp. metK18 and Nocardia sp. CS682 expressing S-adenosylmethionine synthetase (MetK), the production of nargenicin A1 was improved by ~5.57-fold by supplementation with 30 mM methyl oleate, 5.01-fold by supplementation with 15 mM sodium propionate, and 3.64-fold by supplementation with 15 mM sodium acetate. Furthermore, supplementing the culture broth of Nocardia sp. ACC18 and Nocardia sp. CS682 expressing the acetyl-CoA carboxylase complex (AccA2 and AccBE) with 30 mM methyl oleate, 15 mM sodium propionate, or 15 mM sodium acetate resulted in ~6.99-, 6.46-, and 5.58-fold increases, respectively, in nargenicin A1 production. Our overall results showed that among the supplements, methyl oleate was the most effective precursor supporting the highest titers of nargenicin A1 in Nocardia sp. CS682, Nocardia sp. metK18, and Nocardia sp. ACC18.

Original article Effects of salts of organic acids on the survival of Listeria monocytogenes in soft cheeses

Abstract: Summary The purpose of this study was to determine the effect of sodium lactate and sodium propionate, both in combination with sodium acetate, on strains of Listeria monocytogenes in artificially inoculated soft cheeses. Minas Frescal and Coalho cheeses, inoculated with a mix of L. monocytogenes 1 ⁄ 2a and Scott A, underwent two treatments: 2% (w ⁄ v) sodium lactate in combination with 0.25% (w ⁄ v) sodium acetate and 2% (w ⁄ v) sodium propionate in combination with 0.25% (w ⁄ v) sodium acetate. The samples were analysed immediately and after 7 days at 10 � C. The growth of the pathogen was inhibited in cheeses containing the salts of organic acids, and the effects of treatment were statistically significant (P < 0.05). However, there was no difference between the types of treatment applied. Our data demonstrate that the effectiveness of the salts of organic acids depended on the initial concentration of L. monocytogenes and that a higher concentration of the salts is necessary to ensure sustained inactivation of target pathogens because they are weakly antilisterial when the soft cheeses are stored at 10 � C.

Mixture for thermal energy storage and device for heat storage and release using said mixture

Abstract: The present invention refers to a mixture for thermal energy storage and to a device for heat storage and release using such a mixture, which mixture comprises at least one compound selected from a first class consisting of compounds having a melting temperature and a fusion enthalpy equal to or higher than 180°C and 150 MJ/m 3 , respectively, selected from β-lactose, myo-inositol, cellobiose, sodium acetate and sodium propionate, in which the at least one compound always comprises β-lactose or sodium propionate or a mixture thereof, in a total amount higher than 45% by weight with respect to the total weight of the mixture, and one or more compounds selected from a second class consisting of compounds having a melting temperature lower than 180°C, in a total amount greater than 10% by weight with respect to the total weight of the mixture, in which, in the mixture, the compound(s) of the second class are totally miscible, both in solid and liquid phase, with the compound(s) of the first class, the second class consisting of organic compounds made of carbon, hydrogen and oxygen, of sodium salts of carboxylic acids and of potassium salts of carboxylic acids.

Effects of Different Substrate Composition on Biosynthesis of Polyhydroxybutyrate- co -hydroxyvalerate by Recombinant Escherichia coli

Abstract: Cupriavidus necator is well known for its ability to accumulate polyhydroxybutyrate (PHB). When supplemented with propionic acid (or sodium propionate) in the growth medium, the bacterium is also able to synthesize polyhydroxybutyrate-co-hydroxyvalerate (PHBV). In order to increase the fraction of 3-hydroxyvalerate (3HV) in PHBV, we cloned the propionate permease gene prpP from C. necator and the propionyl-CoA synthase gene prpE from Cupriavidus taiwanensis and transformed into an Escherichia coli containing phaCAB operon of C. necator. The effects on PHBV accumulation in cells co-expressed with phaCAB and prpE or prpP in the media contained mixed carbon sources (glucose and sodium propionate) were evaluated. The HV fraction in PHBV increased when prpE or prpP was overexpressed in the cells. Concentrations of yeast extracts could also affect the fraction of HV. In addition, when glucose was replaced by sodium pyruvate, sodium succinate, or sodium gluconate, only PHB were detected in the recombinant strains.

Effects of salts of organic acids on the survival of Listeria monocytogenes in soft cheeses

Abstract: Summary The purpose of this study was to determine the effect of sodium lactate and sodium propionate, both in combination with sodium acetate, on strains of Listeria monocytogenes in artificially inoculated soft cheeses Minas Frescal and Coalho cheeses, inoculated with a mix of L monocytogenes 1/2a and Scott A, underwent two treatments: 2% (w/v) sodium lactate in combination with 025% (w/v) sodium acetate and 2% (w/v) sodium propionate in combination with 025% (w/v) sodium acetate The samples were analysed immediately and after 7 days at 10 °C The growth of the pathogen was inhibited in cheeses containing the salts of organic acids, and the effects of treatment were statistically significant (P < 005) However, there was no difference between the types of treatment applied Our data demonstrate that the effectiveness of the salts of organic acids depended on the initial concentration of L monocytogenes and that a higher concentration of the salts is necessary to ensure sustained inactivation of target pathogens because they are weakly antilisterial when the soft cheeses are stored at 10 °C

Preservative composition and article comprising the same

Abstract: PURPOSE: An antiseptic composition and a product containing the same are provided to be easily applied to products and to en sure antifungal and antibacterial effect. CONSTITUTION: A wet tissue contains an antiseptic composition. The antiseptic composition contains 0.01-40 wt% of chloride cetyl pyridinium; 0.01-40 wt% of sodium dehydroacetic acid; 0.01-30 wt% of sodium benzoate, potassium sorbate, sodium propionate, calcium propionate, glucono delta lactone, or a mixture thereof; and remaining amount of purified water.

Low-cost feed prepared by using micro-ecological additive

Abstract: The invention discloses a low-cost feed prepared by using a micro-ecological additive. The low-cost feed consists of the following components in percentage by weight: 0.1 percent of micro-ecological additive, 0.1 percent of brown sugar, 50 percent of saw dust, 20 to 30 percent of corn flour, 15 to 20 percent of chicken manure, 0.5 to 1 percent of glyceryl monostearate, 1 to 3 percent of sodium propionate, 0.1 to 0.3 percent of anti-mildew agent, and 7 to 10 percent of water. The low-cost feed is prepared by the following steps of: soaking the saw dust into the water for 24 hours, adding the micro-ecological additive and the brown sugar, mixing uniformly, adding the corn flour, the chicken manure, the glyceryl monostearate, the sodium propionate and the anti-mildew agent, and stirring and mixing uniformly; controlling the fermentation temperature at 60+/-5 DEG C, wherein the highest fermentation temperature is not more than 70 DEG C; and performing fermentation for 72 to 100 hours, discharging the product after the product is qualified by analysis, and thus obtaining a feed product. The product is mainly used as concentrated feed.

Water-rinsable dust-sticking film

Abstract: A water-rinsable dust-sticking film is characterized in that the film comprises the following components by mass: 75.0-86.0% of deionized water, 0.5-1.0% of thickening agents, 1.0-2.5% of carboxymethylcellulose sodium, 2.0-5.0% of surfactants, 10.0-15.0% of glycerol, and 0.5-1.5% of preservatives. The thickening agent in the invention is organic bentonites. The surfactant in the invention is polyoxyethylene octylphenol ether and sucrose fatty acid ester. The preservative in the invention is calcium propionate and sodium propionate.

Lyophilized preparation of botulinun toxin

Abstract: PURPOSE: A lyophilized preparation of botulinum toxin without animal protein stabilizing agent is provided to maintain botulinum toxin activation and to ensure excellent long-term stability. CONSTITUTION: A pharmaceutical lyophilized preparation contains botulinum toxin, polysorbate, and methionine; and saccharide, sugar alcohol or ion compound component. The botulinum toxin includes botulinum serotype A, B, C, D, E, F, or G. The polysorbate is polysorbate 20, 40, 60, 80, or 100. The saccharides are trehalose, sucrose, maltose, fructose, lapinose, lactose, or glucose. The sugar alcohol is cyclodextrin, mannitol, sorbitol, glycerol, xylitol, or inositol. The ion compounds are sodium chloride, sodium phosphate, ammonium phosphate, magnesium sulfate, sodium acetate, sodium lactate, sodium succinate, sodium propionate, or potassium phosphate.

The optimization of mildew preventives of soy-based adhesive

Abstract: In order to improve the mouldproof effect of soy-based adhesive,the different mould on the soy-based adhesive surface were separated and identified,and the mouldproof effect of different mildew preventives was researched.The results showed that sodium diacetate,sodium borate,sodium nitrite,potassium sorbet,sodium propionate all had mouldproof effect,when sodium dilacerate and sodium borate dosage were 0.3%,the mouldproof effect was the best.Comprehensive consideration,the anti-fungal soybean fibrin glue was used for mildew treatment,sodium diacetate and sodium borate were the best chooses.

Influence of different carbon sources on nitrogen and phosphorus removal performances of 4 kinds of nitrosation denitrifying phosphorus accumulating organisms

Abstract: In order to understand the needs of carbon sources of NDPAOs thoroughly,the influence of different carbon sources on nitrogen and phosphorus removal performances of Sta,Par,Kleand Bacwere studied using culture-dependent methodThe results showed that,the carbon source which was most likely to be used for the said 4 kinds of NDPAOs was glucoseUnder anaerobic condition,for the said 4 kinds of NDPAOs,the maximal degradation amounts of COD with unit cell could be obtained when glucose was used as the carbon source,which were 39×10-6,35×10-6,22×10-6 and 26×10-6 mg/cfu respectivelyFor Staand Bac,when sodium propionate was used as the carbon source,the amount of phosphate released by them reached the highest,which were 69×10-9 and 62×10-9 mg/cfu respectively;for Par,the highest release amount of phosphate was obtained with sodium acetate and sucrose as the carbon source,which was 40×10-9 mg/cfu;for Kle,the maximal release amount of phosphate could happened when sodium acetate was used as the carbon source,which was 28×10-8 mg/cfuUnder anoxic condition,for Sta,Par,Kleand Bac,the nitrite consumption by them reached the highest when sodium propionate,glucose,glucose,and sodium propionate were respectively used as the carbon source,and the consumption amount were 81×10-8,81×10-8,41×10-8 and 64×10-8 mg/cfu respectively;meanwhile,the amount of phosphate adsorbed by them also reached the highest,which were 15×10-8,13×10-8,96×10-9 and 13×10-8 mg/cfu respectively