Showing papers on "Stigmasterol published in 1996"
TL;DR: In this article, a new oleanane derivative and a stigmasterol glycoside were isolated from the roots of Ambroma augusta and their structures were established as olean-12-en 2β,3β-diol-28-oic acid and stigmast-5,22-dien -3-O-α-d -glucopyranoside respectively, on the basis of full spectral analyses and chemical methods.
79 citations
TL;DR: In this paper, the root level analysis of proteins, phospholipids, total fatty acids, and sterols was performed at root level in the whole tissue extract, in the crude microsomal fraction and in the plasma membrane fraction purified by two-phase partitioning.
Abstract: Soybean seedlings were grown hydroponically in a nutrient medium with or without 25 mM NaCI. An analysis of proteins, phospholipids, total fatty acids, and sterols was performed at the root level in the whole tissue extract, in the crude microsomal fraction and in the plasma membrane fraction purified by two-phase partitioning. The free and conjugated forms of sterols were analysed. Most of the sterols were found in the free form, whereas steryl glycosides (SG) were detected in small quantities and steryl esters (SE) did not occur in the membrane fractions. The compositions in various moieties of sterols were quite similar among the three materials, i.e. sitosterol was the major sterol accompanied by campesterol, stigmasterol and small quantities of cholesterol and isofucosterol. No changes were detected in the whole root tissues after salt treatment. Surprisingly, the main lipid changes were observed in the microsomal fraction where the phospholipid and sterol content decreased by 50%, accompanied by an increasing saturation of the total fatty acids. Only this last parameter was altered in the plasma membrane fraction and to a lesser extent. The saturated fatty acids (16 :0) and (18 :0) which both accounted for 49% of the total fatty acids in the plasma membrane increased 56% under salt stress. The different responses of the lipid classes induced by salt treatment and their possible significance are discussed.
67 citations
TL;DR: The influence of processing on soya bean lipids during kinema pro-duction was studied in this article, where the major fatty acid present in all samples was the essential fatty acid, linoleic acid.
Abstract: The influence of processing on soya bean lipids during kinema pro- duction was studied. The crude lipid (CL), free fatty acid (FFA) and phytosterol contents of raw beans were 121 g, 6.9 g and 0.8 g kg-' dry matter, respectively. After soaking and cooking the moisture content of beans increased significantly from 121 to 749 g kg-', whilst the lipid contents were unchanged. During a 2 day fermentation at 3TC, the lipid contents increased significantly as indicated by capillary column gas chromatography (GC) analysis of petroleum ether extracts of lyophilised samples of unfermented and fermented beans. The fatty acids, palmitic (C16 : 0). stearic (C18 : 0), oleic (C18 : I), linoleic (C18 : 2), linolenic ((218 : 3) and arachidic (C20: 0) acids were identified. The major fatty acid present in all samples was the essential fatty acid, linoleic acid. All of the afore- mentioned fatty acids (except arachidic) were liberated during fermentation by microbial lipases, yielding approximately the same proportion of fatty acids found in unfermented beans. The unsaponifiable soya bean lipids identified by GC were campesterol, stigmasterol and /7-sitosterol (ratio 1.2 : 1 : 3.4). Levels of these increased during fermentation by 50-61 %, but remained in approximately the same proportions.
32 citations
TL;DR: The hexane extract of leaves of Trichilia claussenii yielded the new triterpenoid 24methylene-26-hydroxycycloartan-3-one, 24-methylenecycloartanol fatty acids derivatives, caryophyllene epoxide, a mixture of ω-phenyl alkanoic and alkenoic acids, plastocromenol, α-tocopherol, squalene, and a mixtures of sitosterol and stigmasterol as free alcohols and esterified by fatty
27 citations
TL;DR: The modification of the configuration at C-24 has thus an influence on the sterol ordering ability in the case of a coexistence of the Δ12 double bond and the ethyl group, i.e. in the stigmasterol series.
25 citations
TL;DR: Deuterium NMR on oriented lipid bilayers made of soybean phosphatidylcholine and containing these four labeled plant sterols clearly reveals the difference of orientation and mobility of the four side chains.
Abstract: The plant sterols sitosterol and stigmasterol exert very different effects on plant model membranes, the first one being a “reinforcer” like cholesterol, the second one not. 25-2H-Stigmasterol has been synthesized by coupling of the 22-aldehyde derived from stigmasterol by ozonolysis, with the proper sulfone labeled in position 25. The configuration of the ethyl side chain at C-24 was controlled by separation of the diastereomers introduced via a chiral sulfoxide. This synthetic scheme allowed the introduction of a labeled side chain in plant sterols in eight steps for stigmasterol and nine for sitosterol (overall yield ca. 15%). Using both diastereomers, the 24-epimers of sitosterol (clionasterol) and stigmasterol (poriferasterol) have also been synthesized. Deuterium NMR on oriented lipid bilayers made of soybean phosphatidylcholine and containing these four labeled plant sterols clearly reveals the difference of orientation and mobility of the four side chains.
22 citations
Journal Article•
TL;DR: A mixture of sterols containing and stigmasterol were isolated from the leaves of Gynura procumbens and after acetylation of 2 with pyridine-acetic anhydride, ${\beta}>-sitosterol$ was isolated.
Abstract: A mixture of sterols containing and stigmasterol (1), and sterol glycosides containing and stigmasterol (2) were isolated from the leaves of Gynura procumbens. After acetylation of 2 with pyridine-acetic anhydride, ${\beta}>-sitosterol$ (3) was isolated.
20 citations
TL;DR: Elongatin, 12a-hydroxyrotenone, β-sitosterol and stigmasterol have been isolated and identified from the stems and leaves of Tephrosia uniflora.
Abstract: Elongatin (1), 12a-hydroxyrotenone (5), β-sitosterol and stigmasterol have been isolated and identified from the stems and leaves of Tephrosia uniflora.
11 citations
TL;DR: The phytosteroids 3s-hydroxy-(24S)-stigmast-5, 22E-dien-7-one and (24S)stigmasta-5 (22E-diene-3\, 7s-diol have been synthesized from stigmasterol as discussed by the authors.
Abstract: The phytosteroids 3s-hydroxy-(24S)-stigmast-5, 22E-dien-7-one and (24S)-stigmasta-5, 22E-diene-3\, 7s-diol have been synthesized from stigmasterol.
2 citations
Journal Article•
TL;DR: In this paper, a study about the subtropical fruit was analyzed the chemical composition of the nut Pecan lipidic fraction obtained in experimental fields in South Italy, some biometric, carpologic and merceologic parameters were measured on the nut.
Abstract: In a study about the subtropical fruit was analysed the chemical composition of the nut Pecan lipidic fraction obtained in experimental fields in South Italy. Some biometric, carpologic and merceologic parameters were measured on the nut. The chemical investigation on the oil have interested the following evaluations: organic acidity, peroxide number, unsaponifiable matter, oxidation resistance, spectrophotometric determination in the UV range, triglyceridic, acidic and sterolic composition, tocopherols content. From the analytical results the Pecan flour has demonstrated a high content of oil that presented a good stability to oxidation. The fatty acid composition has a typical pattern of vegetable oil. Palmitic, heptadecanoic, stearic, arachic, and behenic are the saturated acids present; palmitoleic, heptadecenoic, oleic, linoleic, linolenic, and eicosenoic are the unsaturated. The oleic acid has showed the higher value (64.6%) in the Green Rever cultivar, the linoleic acid in the Cheyenne cultivar is very high (35.09%). Very important is the total unsaturated fatty acids that in the Green Rever is higher than 91%. The triglyceride profile is represented of fifteen triglycerides, the mix triacylglycerols are the prevalent and the dilinoleyloleylglycerol showed the highest value in the cultivar Cheyenne with a chromatographic area ratio of 29.38%, measured by an UV detector settled at 210 nm. The sterolic composition is the following: cholesterol, brassicasterol, campesterol, stigmasterol, clerosterol, β-sitosterol, Δ 5 -avenasterol, Δ 5,24 -stigmasterol, Δ 7 -stigmastenol, Δ 7 -avenasterol. The tocopherol content is the highest in the Mohawk cv (392 ppm) and the lowest in the Stuart (88 ppm).
1 citations
Journal Article•
TL;DR: In this article, seven compounds were isolated from the roots of Polyyala arillata Buch-Ham Onbasis of chemical reactions and spectral (UV) IR,MS,1 HNMR,DIFNoE,13CNMR) analysis, they were identified as 1-methoxy-2,3-methylenedioxyxanthone(I), 1-7-dihydroxy-2.3-methylene-dioxyxianthone (II), 1,6,7-trihydroxy- 2,3dimeth
Abstract: Seven compounds were isolated from the roots of Polyyala arillata Buch-Ham Onbasis of chemical reactions and spectral (UV) IR ,MS,1 HNMR,DIFNoE,13CNMR)analysis, theywere identified as 1-methoxy-2,3-methylenedioxyxanthone(I),1,7-dihydroxy-2,3-methylene-dioxyxanthone(II),1,6,7-trihydroxy-2,3-dimethoxyxanthone(III),p-hydroxy-benzoic acid(IV),polygalitol(V),stigmasterol(VI)and stigmasterol-3-O-β-D-glucopyranoside(VII)II and IIIare new compoundsPharmacological study indicated that I,Il and III inhibit aldose reductase