Showing papers on "Totipotent published in 1990"
TL;DR: It is demonstrated that single stem cell clones are sufficient to maintain hematopoiesis over the lifetime of an animal and suggested that mono- or oligoclonality may be a hallmark of long-term reconstituted systems.
Abstract: We have analyzed the temporal in vivo fate of 142 individual stem cell clones in 63 reconstituted mice. Long-term sequential analyses of the four major peripheral blood lineages, obtained from animals engrafted with genetically marked stem cells, indicate that developmental behavior is primarily a function of time. As such, the first 4-6 months post-engraftment is characterized by frequent fluctuations in stem cell proliferation and differentiation behavior. Gradually, a stable hematopoietic system emerges, dominated by a small number of totipotent clones. We demonstrate that single stem cell clones are sufficient to maintain hematopoiesis over the lifetime of an animal and suggest that mono- or oligoclonality may be a hallmark of long-term reconstituted systems. A model is proposed, wherein lineage-restricted differentiation and dramatic clonal flux are consequences of mechanisms acting on an expanding pool of totipotent cells and are not indicative of intrinsically distinct stem cell classes.
574 citations
TL;DR: The fetal totipotent hematopoietic stem cell is characterized using a novel strategy that integrates physical analysis of cell properties and genetic analysis of in vivo developmental behavior and allows the simultaneous isolation and in vivo characterization of any stem cell population.
303 citations
TL;DR: The feasibility of retroviral-marking techniques is established to demonstrate the maintenance of totipotent lympho-myeloid stem cells for at least 4 weeks in the long- term marrow culture system and provide the first evidence of their proliferation in vitro.
103 citations
TL;DR: The manipulation of plant growth regulator concentration is probably the most widely used technique for the induction of organogenesis, and this methodology has formed the basis of the propagation of commercially important plants via tissue culture in recent years.
Abstract: Plant cells can be totipotent, i.e., each cell may be capable of developing into an entire plant when provided with the correct environmental stimuli. Research during the last 30 yr has demonstrated that successful organogenesis in callus cultures can be achieved by the correct choice of medium components, selection of a suitable inoculum, and control of the physical environment (1). The manipulation of plant growth regulator concentration is probably the most widely used technique for the induction of organogenesis, and this methodology has formed the basis of the propagation of commercially important plants via tissue culture in recent years (2).
9 citations
Journal Article•
1 citations