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A.A.M. Gribnau
Researcher at Radboud University Nijmegen
Publications - 4
Citations - 178
A.A.M. Gribnau is an academic researcher from Radboud University Nijmegen. The author has contributed to research in topics: RNase P & Sephadex. The author has an hindex of 4, co-authored 4 publications receiving 178 citations.
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Journal ArticleDOI
Purification of rat liver RNase inhibitor and its effect on polyribosome integrity.
TL;DR: The RNase inhibitor from 100,000 g rat liver supernatant fraction has been purified 3000–4000-fold by ammonium sulfate fractionation, DEAE-Sephadex A-50 chromatography, and gel filtration on SephadeX G-100 to stabilize the polysomal profile on sucrose gradients.
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Further studies on the ribonuclease inhibitor from rat liver: Stability and other properties
TL;DR: The enzyme-inhibitor complex is formed instantaneously and is strongly dependent on pH and Mg 2+ concentration, and the pH optimum of the inhibitor coincides with the optimal pH value of pancreatic ribonuclease.
Journal ArticleDOI
High purification of the RNase inhibitor from rat liver by affinity chromatography.
TL;DR: A highly purified RNase inhibitor from rat liver can be obtained by chromatography on columns loaded with carboxymethyl cellulose-RNase and is now available for the preparation of undegraded polyribosomes and ribosomal subunits.
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Proteolytic activity of partly purified ribonuclease inhibitor from rat liver
TL;DR: Evidence is provided that at least part of the enzymic activity has to be ascribed to a potent leucine aminopeptidase that can be removed by gel filtration on Sephadex G-100.